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<p>Strain Y3<sup>T</sup>, a Gram-stain-negative, aerobic, non-motile rod capable of producing yellow pigments, was isolated from the intestinal tract of <i>Scomberomorus niphonius</i> collected in Fangchenggang, Guangxi Province, China (21° 33′ 11.6″ N, 108° 25′ 42.5″ E). Optimal growth conditions were observed at 30&#xa0;°C and pH 7.0 in the presence of 1% (w/v) NaCl. Phylogenetic analysis based on the 1478 bp sequence of the 16S rRNA gene revealed that strain Y3<sup>T</sup> belongs to the genus <i>Chryseobacterium</i> (family <i>Weeksellaceae</i>), exhibiting the highest sequence similarity to <i>Chryseobacterium buanense</i> DSM 22323<sup>T</sup> (98.69%) and <i>Soonwooa purpurea</i> JCM 31880<sup>T</sup> (98.10%). The draft genome size was determined to be 2,806,243 bp with a DNA G+C content of 35.2%. Digital DNA-DNA hybridisation and average nucleotide identity values between strain Y3<sup>T</sup> and its closely related species were 19.9–44.1% and 70.29–91.23%, respectively. The predominant fatty acids were identified to be iso-C<sub>15:0</sub> (51.6%) and iso-C<sub>17:0</sub> 3-OH (14.3%). Menaquinone-6 (MK-6) was identified as the sole respiratory quinone in this strain. Polar lipid analysis revealed the presence of phosphatidylethanolamine, four unidentified lipids, two unidentified glycolipids, and an unidentified aminolipid. Strain Y3<sup>T</sup> demonstrated capabilities for nitrate and nitrite reduction, which is corroborated by the identification of denitrification-related genes within its genome. Based on comprehensive phylogenetic, phenotypic, chemotaxonomic, and genomic analyses, strain Y3<sup>T</sup> is proposed as the type strain of a novel species, designated as <i>Chryseobacterium intestini</i> sp. nov. The type strain is Y3<sup>T</sup> (= JCM 37830<sup>T</sup> = GDMCC 1.5522<sup>T</sup>).</p>

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Chryseobacterium intestini sp. nov., a denitrifying bacterium isolated from the intestine of the Spanish mackerel, Scomberomorus niphonius

  • 期刊论文

  • 发表日期：

    2026年5月18日

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  • Yuxin Zhang,
  • Yilin Ren,
  • Jiling Li,
  • Doudou Yin,
  • Hongyan Zhang,
  • Naikun Shen

摘要

Strain Y3T, a Gram-stain-negative, aerobic, non-motile rod capable of producing yellow pigments, was isolated from the intestinal tract of Scomberomorus niphonius collected in Fangchenggang, Guangxi Province, China (21° 33′ 11.6″ N, 108° 25′ 42.5″ E). Optimal growth conditions were observed at 30 °C and pH 7.0 in the presence of 1% (w/v) NaCl. Phylogenetic analysis based on the 1478 bp sequence of the 16S rRNA gene revealed that strain Y3T belongs to the genus Chryseobacterium (family Weeksellaceae), exhibiting the highest sequence similarity to Chryseobacterium buanense DSM 22323T (98.69%) and Soonwooa purpurea JCM 31880T (98.10%). The draft genome size was determined to be 2,806,243 bp with a DNA G+C content of 35.2%. Digital DNA-DNA hybridisation and average nucleotide identity values between strain Y3T and its closely related species were 19.9–44.1% and 70.29–91.23%, respectively. The predominant fatty acids were identified to be iso-C15:0 (51.6%) and iso-C17:0 3-OH (14.3%). Menaquinone-6 (MK-6) was identified as the sole respiratory quinone in this strain. Polar lipid analysis revealed the presence of phosphatidylethanolamine, four unidentified lipids, two unidentified glycolipids, and an unidentified aminolipid. Strain Y3T demonstrated capabilities for nitrate and nitrite reduction, which is corroborated by the identification of denitrification-related genes within its genome. Based on comprehensive phylogenetic, phenotypic, chemotaxonomic, and genomic analyses, strain Y3T is proposed as the type strain of a novel species, designated as Chryseobacterium intestini sp. nov. The type strain is Y3T (= JCM 37830T = GDMCC 1.5522T).