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<p>A comprehensive polyphasic investigation was conducted on a novel verrucomicrobium, designated as 23ND18S-11<sup>T</sup>, isolated from riverside soil in South Korea. Strain 23ND18S-11<sup>T</sup> was coccoid-shaped that occur predominantly in pairs, Gram-stain-negative, obligately aerobic, pale yellow-pigmented, and non-motile. It grew at 0–1.0% NaCl (w/v), pH 6.0–9.5, and 20–30&#xa0;°C. Strain 23ND18S-11<sup>T</sup> was assigned to the family <i>Opitutaceae</i> in the phylum <i>Verrucomicrobiota</i>, showing the highest 16S rRNA gene sequence similarity (95.0%) to <i>Horticoccus luteus</i> KSB-15<sup>T</sup>. MK-7 was the sole quinone and the predominant (&gt; 10%) fatty acids were anteiso-C<sub>15:0</sub> and C<sub>16:0</sub>. The polar lipids were composed of phosphatidylethanolamine, two unidentified aminophospholipids, an unidentified glycolipid, four unidentified glycophospholipids, and an unidentified lipid. The genome size of strain 23ND18S-11<sup>T</sup> was 7.2&#xa0;Mb with a genomic DNA G + C content of 65.5%. The AAI and POCP values between the genomes of strain 23ND18S-11<sup>T</sup> and its closely related taxa in the family <i>Opitutaceae</i> were 63.7–67.6% and 31.8–48.6%, respectively. The <i>d</i>DDH value of strain 23ND18S-11<sup>T</sup> with <i>H. luteus</i> KSB-15<sup>T</sup> was 19.7%. The genome of strain 23ND18S-11<sup>T</sup> was found to contain a cluster of genes (<i>oleA</i>, <i>oleB</i>, <i>oleC</i>, and <i>oleD</i>) involved in alkene synthesis as well as several genes that encode industrially significant enzymes involved in the degradation of bio-macromolecules. Consequently, the strain 23ND18S-11<sup>T</sup> should be identified and classified as a novel genus and species of the family <i>Opitutaceae</i>, for which the name <i>Terrisphaera buseokensis </i>gen. nov., sp. nov. is proposed. The type strain is 23ND18S-11<sup>T</sup> (= KCCM 90593<sup>T</sup> = FBCC-B19162<sup>T</sup> = NBRC 117123<sup>T</sup>). </p>

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Phylogenomic and phenotypic investigations of Terrisphaera buseokensis gen. nov., sp. nov., a verrucomicrobium isolated from riverside soil

  • 期刊论文

  • 发表日期：

    2026年5月16日

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  • Jaewoo Yoon,
  • Seong-Ryong Lee,
  • Sanghwa Park

摘要

A comprehensive polyphasic investigation was conducted on a novel verrucomicrobium, designated as 23ND18S-11T, isolated from riverside soil in South Korea. Strain 23ND18S-11T was coccoid-shaped that occur predominantly in pairs, Gram-stain-negative, obligately aerobic, pale yellow-pigmented, and non-motile. It grew at 0–1.0% NaCl (w/v), pH 6.0–9.5, and 20–30 °C. Strain 23ND18S-11T was assigned to the family Opitutaceae in the phylum Verrucomicrobiota, showing the highest 16S rRNA gene sequence similarity (95.0%) to Horticoccus luteus KSB-15T. MK-7 was the sole quinone and the predominant (> 10%) fatty acids were anteiso-C15:0 and C16:0. The polar lipids were composed of phosphatidylethanolamine, two unidentified aminophospholipids, an unidentified glycolipid, four unidentified glycophospholipids, and an unidentified lipid. The genome size of strain 23ND18S-11T was 7.2 Mb with a genomic DNA G + C content of 65.5%. The AAI and POCP values between the genomes of strain 23ND18S-11T and its closely related taxa in the family Opitutaceae were 63.7–67.6% and 31.8–48.6%, respectively. The dDDH value of strain 23ND18S-11T with H. luteus KSB-15T was 19.7%. The genome of strain 23ND18S-11T was found to contain a cluster of genes (oleA, oleB, oleC, and oleD) involved in alkene synthesis as well as several genes that encode industrially significant enzymes involved in the degradation of bio-macromolecules. Consequently, the strain 23ND18S-11T should be identified and classified as a novel genus and species of the family Opitutaceae, for which the name Terrisphaera buseokensis gen. nov., sp. nov. is proposed. The type strain is 23ND18S-11T (= KCCM 90593T = FBCC-B19162T = NBRC 117123T).