<p>Airborne microbes from animal confinement facilities not only amplify the risk of disease spread among livestock but also pose substantial health threats to animals and farm workers. The objective of this study was to investigate the microbial counts in cattle sheds and their relationship with meteorological factors, including temperature, relative humidity, and air velocity, as well as microbial diversity. Sampling was carried out both indoors and outdoors of two cattle sheds throughout three seasons (summer, rainy, and winter), at fortnightly intervals. Results showed that bacterial and fungi counts ranged from 0.0 to 1.60 × 10³&#xa0;CFU/m³ inside the sheds and from 0.0 to 1.08 × 10³&#xa0;CFU/m³ outside, with significant variation between areas and seasons. The predominant microbial count was mesophilic bacteria followed by staphylococci, fungi and <i>Enterobacteriaceae</i>. The mesophilic bacteria, <i>Enterobacteriaceae</i> and fungi showed statistically significant positive correlation with air temperature while air velocity with <i>Enterobacteriaceae</i> bacteria. No significant correlation exists between relative humidity and microbial concentration. The bacterial families <i>Staphylococcaceae</i> and <i>Bacillaceae</i> from the mesophilic group of bacteria were identified as the most prevalent, whereas the dominant fungi taxa in the cattle sheds were <i>Aspergillus</i> spp. and <i>Penicillium</i> spp. The microbial environment within cattle sheds under loose housing systems was found to be well-regulated, with airborne bacteria and fungi levels remaining within recommended limits.</p>