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<p>A novel polysaccharide-degrading bacterial strain, designated ASW11-125<sup>T</sup>, was isolated from intertidal sediments in Aoshan Bay, Qingdao, China. The strain was strictly aerobic, Gram-stain-negative, catalase-positive but oxidase-negative, short rod-shaped, and exhibited gliding motility without flagella. Growth occurred at 4–35°C (optimum 28°C), pH 6.0–8.0 (optimum pH 7.0), and in 0.5–16.0% NaCl (optimum 2.5–3.0%). The predominant polar lipid was phosphatidylethanolamine. Major fatty acids were iso-C<sub>15:0</sub> and iso-C<sub>17:0</sub> 3-OH, and the primary respiratory quinone was menaquinone-6 (MK-6). Based on the phylogenetic analyses of 16S rRNA gene sequences and 1542 single copy orthologous clusters, strain ASW11-125<sup>T</sup> affiliated with the genus <i>Christiangramia</i> and was closely related to <i>Christiangramia portivictoriae</i> MCCC 1A00585<sup>T</sup> (98.8%), <i>Christiangramia aquimixticola</i> KCTC 42706<sup>T</sup> (98.8%) and <i>Christiangramia marina</i> KCTC 12366<sup>T</sup> (98.6%). Comparative genomic analysis revealed that the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain ASW11-125<sup>T</sup> and its closely related species were 74.6–91.5% and 18.6–44.3%, respectively, which were below the cutoff values for proposing a novel species. Based on a polyphasic characterization integrating phenotypic, phylogenetic, and chemotaxonomic evidence, strain ASW11-125<sup>T</sup> represents a novel species of the genus <i>Christiangramia</i>, for which the name <i>Christiangramia qingdaonensis</i> sp. nov. is proposed. The draft genome of strain ASW11-125<sup>T</sup> is 3.2&#xa0;Mb in size with a G + C content of 38.3%. Notably, genomic analysis revealed an abundance of genes encoding putative carbohydrate-active enzymes (CAZymes), particularly those associated with starch, laminarin, and fructan utilization, suggesting its potential role in the marine carbon cycle. The type strain is ASW11-125<sup>T</sup> (= KCTC 102340<sup>T</sup> = MCCC 1K09555<sup>T</sup>).</p>

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Christiangramia qingdaonensis sp. nov., a novel polysaccharide-degrading Bacteroidota bacterium, isolated from intertidal sediment

  • 期刊论文

  • 发表日期：

    2026年1月6日

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  • Zhao-Tong Chen,
  • Bing Yu,
  • Yu-Shan Li,
  • Zhi-Wei Zhu,
  • Xi-Ying Zhang,
  • Yi Li

摘要

A novel polysaccharide-degrading bacterial strain, designated ASW11-125T, was isolated from intertidal sediments in Aoshan Bay, Qingdao, China. The strain was strictly aerobic, Gram-stain-negative, catalase-positive but oxidase-negative, short rod-shaped, and exhibited gliding motility without flagella. Growth occurred at 4–35°C (optimum 28°C), pH 6.0–8.0 (optimum pH 7.0), and in 0.5–16.0% NaCl (optimum 2.5–3.0%). The predominant polar lipid was phosphatidylethanolamine. Major fatty acids were iso-C15:0 and iso-C17:0 3-OH, and the primary respiratory quinone was menaquinone-6 (MK-6). Based on the phylogenetic analyses of 16S rRNA gene sequences and 1542 single copy orthologous clusters, strain ASW11-125T affiliated with the genus Christiangramia and was closely related to Christiangramia portivictoriae MCCC 1A00585T (98.8%), Christiangramia aquimixticola KCTC 42706T (98.8%) and Christiangramia marina KCTC 12366T (98.6%). Comparative genomic analysis revealed that the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain ASW11-125T and its closely related species were 74.6–91.5% and 18.6–44.3%, respectively, which were below the cutoff values for proposing a novel species. Based on a polyphasic characterization integrating phenotypic, phylogenetic, and chemotaxonomic evidence, strain ASW11-125T represents a novel species of the genus Christiangramia, for which the name Christiangramia qingdaonensis sp. nov. is proposed. The draft genome of strain ASW11-125T is 3.2 Mb in size with a G + C content of 38.3%. Notably, genomic analysis revealed an abundance of genes encoding putative carbohydrate-active enzymes (CAZymes), particularly those associated with starch, laminarin, and fructan utilization, suggesting its potential role in the marine carbon cycle. The type strain is ASW11-125T (= KCTC 102340T = MCCC 1K09555T).