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<p>A short rod-shaped, Gram-negative, strictly aerobic, non-motile bacterium, designated as NW5<sup>T</sup>, was isolated from a eutrophic pond in Jimo District, Qingdao, Shandong Province, China. The strain exhibited growth between 20–42 °C (optimal at 30 °C), pH 6.0–10.0 (optimal at pH 8.0), and 0–2.0% (w/v) NaCl (optimal at 0% NaCl). Based on 16S rRNA gene sequence analysis, strain NW5<sup>T</sup> showed the highest similarity with ‘<i>Pseudomonas oryzae</i>’ CGMCC 1.12417<sup>T</sup> (98.2%), followed by <i>Pseudomonas oryzagri</i> CGMCC 1.12618<sup>T</sup> (97.8%), and <i>Pseudomonas guguanensis</i> JCM 18416<sup>T</sup> (97.7%). Phylogenetic analysis based on 16S rRNA gene sequence and whole-genome sequence indicated that strain NW5<sup>T</sup> belongs to the genus <i>Pseudomonas</i>. The genome size of strain NW5<sup>T</sup> is approximately 2.6 Mb with a DNA G+C content of 65.3%, which is one of the smallest genomes reported to date among all <i>Pseudomonas</i> type strains. The average nucleotide identity and digital DNA-DNA hybridization values between strain NW5<sup>T</sup> and the closely related type strains were in the range of 75.8–87.2% and 21.4–26.6%, respectively, which were below the species delineation thresholds. The major fatty acids (≥5.0%) were C<sub>10:0</sub> 3-OH, C<sub>12:0</sub>, C<sub>16:0</sub>, summed feature 3 and summed feature 8. The predominant isoprenoid quinone was ubiquinone-8 (Q-8) and small amounts of Q-7 and Q-9 were also detected, which is quite different from the ubiquinone profile of <i>Pseudomonas</i>. Strain NW5<sup>T</sup> harbored a relatively large number of genes in the metabolic pathway for multi-xenobiotics degradation. In addition, strain NW5<sup>T</sup> was highly resistant to zinc (100 mM), consistent with the genotype. Based on the phenotypic, phylogenetic, chemotaxonomic, and physiological analyses, strain NW5<sup>T</sup> is considered to represent a novel species of the genus <i>Pseudomonas</i>, for which the name <i>Pseudomonas eutrophicaquae</i> sp. nov. is proposed. The type strain is NW5<sup>T</sup> (=GDMCC 1.3065<sup>T</sup> =MCCC 1K07223<sup>T</sup> =JCM 35388<sup>T</sup>).</p>

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Pseudomonas eutrophicaquae sp. nov., a novel xenobiotic-degrading non-motile bacterium, containing Q-8 ubiquinone, isolated from an aquatic ecosystem in Jimo District, China

  • 期刊论文

  • 发表日期：

    2026年1月13日

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  • Kaiyue Wang,
  • Jinhua Hu,
  • Ke Ma,
  • Haoran Liu,
  • Doudou Kang,
  • Mengyang Li,
  • Jianjun Song,
  • Bai Guo,
  • Shuzhen Wei,
  • Aijv Liu,
  • Rui Zhang,
  • Fengtang Yang,
  • Hongliang Liu

摘要

A short rod-shaped, Gram-negative, strictly aerobic, non-motile bacterium, designated as NW5T, was isolated from a eutrophic pond in Jimo District, Qingdao, Shandong Province, China. The strain exhibited growth between 20–42 °C (optimal at 30 °C), pH 6.0–10.0 (optimal at pH 8.0), and 0–2.0% (w/v) NaCl (optimal at 0% NaCl). Based on 16S rRNA gene sequence analysis, strain NW5T showed the highest similarity with ‘Pseudomonas oryzae’ CGMCC 1.12417T (98.2%), followed by Pseudomonas oryzagri CGMCC 1.12618T (97.8%), and Pseudomonas guguanensis JCM 18416T (97.7%). Phylogenetic analysis based on 16S rRNA gene sequence and whole-genome sequence indicated that strain NW5T belongs to the genus Pseudomonas. The genome size of strain NW5T is approximately 2.6 Mb with a DNA G+C content of 65.3%, which is one of the smallest genomes reported to date among all Pseudomonas type strains. The average nucleotide identity and digital DNA-DNA hybridization values between strain NW5T and the closely related type strains were in the range of 75.8–87.2% and 21.4–26.6%, respectively, which were below the species delineation thresholds. The major fatty acids (≥5.0%) were C10:0 3-OH, C12:0, C16:0, summed feature 3 and summed feature 8. The predominant isoprenoid quinone was ubiquinone-8 (Q-8) and small amounts of Q-7 and Q-9 were also detected, which is quite different from the ubiquinone profile of Pseudomonas. Strain NW5T harbored a relatively large number of genes in the metabolic pathway for multi-xenobiotics degradation. In addition, strain NW5T was highly resistant to zinc (100 mM), consistent with the genotype. Based on the phenotypic, phylogenetic, chemotaxonomic, and physiological analyses, strain NW5T is considered to represent a novel species of the genus Pseudomonas, for which the name Pseudomonas eutrophicaquae sp. nov. is proposed. The type strain is NW5T (=GDMCC 1.3065T =MCCC 1K07223T =JCM 35388T).