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<p>A novel Gram stain–positive, endospore-forming, motile, rod-shaped, aerobic bacterial strain, designated PS06<sup>T</sup>, was isolated from the rhizosphere soil of <i>Stipa breviflora</i> at the Siziwang Banner Research Station in Inner Mongolia, PR China. The strain could grow at 4–40&#xa0;℃ (optimum, 37&#xa0;℃), at pH 6.0–9.5 (optimum, pH 8.5), and in the presence of 0–5% NaCl (optimum, 1%). The phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PS06<sup>T</sup> was most closely related to <i>Litchfieldia salsus</i> IBRC-M 10078<sup>T</sup> (16S rRNA similarity, 97.73%) and <i>L. alkalitelluris</i> DSM 16976<sup>T</sup> (97.65%) and formed an independent clade with these two type species in the genus <i>Litchfieldia</i>. The genome analysis showed that the average nucleotide identity (ANI) values and digital DNA–DNA hybridization (dDDH) of strain PS06<sup>T</sup> with <i>L. salsus</i> IBRC-M 10078<sup>T</sup> and <i>L. alkalitelluris</i> DSM 16976<sup>T</sup> were 78.0 and 76.1%, and 21.5 and 22.1%, respectively, which were significantly lower than the thresholds of 95% for ANI and 70% for dDDH for species delineation. The predominant cellular fatty acids (&gt; 10%) were anteiso-C<sub>15:0</sub> and iso-C<sub>15:0</sub>. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and five unknown lipids. The genomic G + C content of strain PS06<sup>T</sup> was 37&#xa0;mol%. Based on the phenotypic, genotypic, chemotaxonomic, and phylogenetic analyses, strain PS06<sup>T</sup> was classified as a novel species of the genus <i>Litchfieldia</i>, and the species name proposed was <i>L. stipae</i> sp. nov. The type strain of the proposed novel species was PS06<sup>T</sup> (= KCTC 43244<sup>T</sup> = CGMCC 1.17355<sup>T</sup>).</p>

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Litchfieldia stipae sp. nov., a novel plant growth–promoting potential bacterium, isolated from the rhizosphere soil of Stipa breviflora in Inner Mongolia, China

  • 期刊论文

  • 发表日期：

    2026年1月3日

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  • Chaojun Wang,
  • Jiajia Shi,
  • Huiqin Yuan,
  • Yunze Bai,
  • Lingling Chen,
  • Meng Zhang,
  • Jiquan Sun,
  • JiFei Xu,
  • Zhihua Bao

摘要

A novel Gram stain–positive, endospore-forming, motile, rod-shaped, aerobic bacterial strain, designated PS06T, was isolated from the rhizosphere soil of Stipa breviflora at the Siziwang Banner Research Station in Inner Mongolia, PR China. The strain could grow at 4–40 ℃ (optimum, 37 ℃), at pH 6.0–9.5 (optimum, pH 8.5), and in the presence of 0–5% NaCl (optimum, 1%). The phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PS06T was most closely related to Litchfieldia salsus IBRC-M 10078T (16S rRNA similarity, 97.73%) and L. alkalitelluris DSM 16976T (97.65%) and formed an independent clade with these two type species in the genus Litchfieldia. The genome analysis showed that the average nucleotide identity (ANI) values and digital DNA–DNA hybridization (dDDH) of strain PS06T with L. salsus IBRC-M 10078T and L. alkalitelluris DSM 16976T were 78.0 and 76.1%, and 21.5 and 22.1%, respectively, which were significantly lower than the thresholds of 95% for ANI and 70% for dDDH for species delineation. The predominant cellular fatty acids (> 10%) were anteiso-C15:0 and iso-C15:0. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and five unknown lipids. The genomic G + C content of strain PS06T was 37 mol%. Based on the phenotypic, genotypic, chemotaxonomic, and phylogenetic analyses, strain PS06T was classified as a novel species of the genus Litchfieldia, and the species name proposed was L. stipae sp. nov. The type strain of the proposed novel species was PS06T (= KCTC 43244T = CGMCC 1.17355T).