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<p>A white, Gram-negative, facultatively anaerobic, floc-forming bacterial strain with the ability of nitrate reduction, designated M4Q-1<sup>T</sup>, was isolated from influent samples of the air flotation tank at a distillery effluent treatment plant. Strain M4Q-1<sup>T</sup> was found to be a non-motile short rod or coccobacillus, oxidase-positive and catalase-negative. Growth was observed at 16–43 °C (optimum 37–40 °C), pH 4.5–8.0 (optimum 7.0), but not in 1.5% (w/v) NaCl (optimum 0%). The sole respiratory quinone of strain M4Q-1<sup>T</sup> was ubiquinone-8 (Q-8), and the major polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), aminophospholipid (APL), phosphatidylglycerol (PG), two unknown phospholipid (PL) and one unidentified polar lipid (L). The predominant fatty acids (&gt; 10% of total fatty acids) were summed feature 3 (C<sub>16:1</sub> <i>ω</i>7<i>c</i> and/or C<sub>16:1</sub> <i>ω</i>6<i>c</i>), summed feature 8 (C<sub>18:1</sub> <i>ω</i>6<i>c</i> and/or C<sub>18:1</sub> <i>ω</i>7<i>c</i>) and C<sub>16:0</sub>. Strain M4Q-1<sup>T</sup> exhibited the highest 16S rRNA gene sequence similarity to the type strain of <i>Brachymonas denitrificans</i> AS-P1<sup>T</sup> (96.90%). Phylogenetic analysis based on 16S rRNA gene sequence and the whole-genome sequences revealed that strain M4Q-1<sup>T</sup> formed a distinct clade closely neighboring the members of the genus <i>Brachymonas.</i> The G + C content of genomic DNA was 64.5&#xa0;mol%. Combined with the analyses of the orthologous average nucleotide identities (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH), strain M4Q-1<sup>T</sup> represented a novel species of the genus <i>Brachymonas</i>, for which the name <i>Brachymonas moutaii</i> sp. nov. is proposed, with the type strain M4Q-1<sup>T</sup> (= MCCC 1K10055 = KCTC 18263).</p>

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Brachymonas moutaii sp. nov., a floc-forming bacterium isolated from a distillery wastewater treatment plant

  • 期刊论文

  • 发表日期：

    2026年1月6日

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  • Yaqi Liu,
  • Hui Wang,
  • Ansi Jiang,
  • Junmei Wu,
  • Bi Chen,
  • Yong Zhang,
  • Siliang Yuan,
  • Yan Huang,
  • Haiyan Zeng,
  • Xin Wang,
  • Dian Jiao,
  • Wei Li,
  • Yuhang Zhu,
  • Dongru Qiu,
  • Ping Xiang

摘要

A white, Gram-negative, facultatively anaerobic, floc-forming bacterial strain with the ability of nitrate reduction, designated M4Q-1T, was isolated from influent samples of the air flotation tank at a distillery effluent treatment plant. Strain M4Q-1T was found to be a non-motile short rod or coccobacillus, oxidase-positive and catalase-negative. Growth was observed at 16–43 °C (optimum 37–40 °C), pH 4.5–8.0 (optimum 7.0), but not in 1.5% (w/v) NaCl (optimum 0%). The sole respiratory quinone of strain M4Q-1T was ubiquinone-8 (Q-8), and the major polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), aminophospholipid (APL), phosphatidylglycerol (PG), two unknown phospholipid (PL) and one unidentified polar lipid (L). The predominant fatty acids (> 10% of total fatty acids) were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) and C16:0. Strain M4Q-1T exhibited the highest 16S rRNA gene sequence similarity to the type strain of Brachymonas denitrificans AS-P1T (96.90%). Phylogenetic analysis based on 16S rRNA gene sequence and the whole-genome sequences revealed that strain M4Q-1T formed a distinct clade closely neighboring the members of the genus Brachymonas. The G + C content of genomic DNA was 64.5 mol%. Combined with the analyses of the orthologous average nucleotide identities (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH), strain M4Q-1T represented a novel species of the genus Brachymonas, for which the name Brachymonas moutaii sp. nov. is proposed, with the type strain M4Q-1T (= MCCC 1K10055 = KCTC 18263).