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<p>A Gram-stain-negative, halophilic, rod-shaped, aerobic bacterial strain, designated as CXY002<sup>T</sup>, was isolated from intertidal seawater of the East China Sea. Strain CXY002<sup>T</sup> exhibited growth at 4–33&#xa0;°C (optimum 28&#xa0;°C), pH 5.5–10 (optimum pH 6–7), and 2–10% (w/v) NaCl (optimum 6%). Based on 16S rRNA gene sequence phylogenetic analysis, strain CXY002<sup>T</sup> exhibited sequence similarities of 98.20% with <i>Maribacter orientalis</i> DSM 16471<sup>T</sup>, 98.12% with <i>M. spongiicola</i> W15M10<sup>T</sup>, 98.00% with <i>M. stanieri</i> DSM 19891<sup>T</sup>, 98.00% with <i>M. cobaltidurans</i> B1<sup>T</sup>, and 97.72% with <i>M. aquivivus</i> DSM 16478<sup>T</sup>. Phylogenomic reconstruction confirmed its affiliation within the genus <i>Maribacter</i>. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between CXY002<sup>T</sup> and closely related species ranged from 15.20 to 16.30% and 71.90 to 72.46%, respectively, both below the recommended thresholds for species demarcation (70% for dDDH and 95–96% for ANI). The genomic DNA G+C content was determined to be 36.5&#xa0;mol%. The predominant respiratory quinone was menaquinone-6 (MK-6), while the major fatty acids comprised iso-C<sub>15:0</sub> (26.7%), 3-OH iso-C<sub>17:0</sub> (19.9%), and iso-C<sub>15:1</sub> (16.6%). Polar lipids included phosphatidylethanolamine, two unidentified aminolipids, and four unidentified lipids. Genomic annotation identified complete carotenoid biosynthesis pathways (<i>crt-B</i>, <i>crt-I</i>, <i>crt-Z</i>), supporting which may contribute to oxidative stress resistance via β-carotene and zeaxanthin biosynthesis. Based on polyphasic taxonomic evidence encompassing phenotypic, chemotaxonomic, and genomic characteristics, strain CXY002<sup>T</sup> represents a novel species within the genus <i>Maribacter</i>, for which the name <i>Maribacter luteocoastalis</i> sp. nov. is proposed. The type strain is CXY002<sup>T</sup> (= MCCC 1K09937<sup>T</sup> = KCTC 102438<sup>T</sup>).</p>

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Maribacter luteocoastalis sp. nov., a novel marine bacterium isolated from the tidal flat sediments of the East China Sea

  • 期刊论文

  • 发表日期：

    2025年8月13日

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  • Xingyu Cao,
  • Tianqiang Bao,
  • Jiaxin Wang,
  • Xi Yu,
  • Jiasong Fang

摘要

A Gram-stain-negative, halophilic, rod-shaped, aerobic bacterial strain, designated as CXY002T, was isolated from intertidal seawater of the East China Sea. Strain CXY002T exhibited growth at 4–33 °C (optimum 28 °C), pH 5.5–10 (optimum pH 6–7), and 2–10% (w/v) NaCl (optimum 6%). Based on 16S rRNA gene sequence phylogenetic analysis, strain CXY002T exhibited sequence similarities of 98.20% with Maribacter orientalis DSM 16471T, 98.12% with M. spongiicola W15M10T, 98.00% with M. stanieri DSM 19891T, 98.00% with M. cobaltidurans B1T, and 97.72% with M. aquivivus DSM 16478T. Phylogenomic reconstruction confirmed its affiliation within the genus Maribacter. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between CXY002T and closely related species ranged from 15.20 to 16.30% and 71.90 to 72.46%, respectively, both below the recommended thresholds for species demarcation (70% for dDDH and 95–96% for ANI). The genomic DNA G+C content was determined to be 36.5 mol%. The predominant respiratory quinone was menaquinone-6 (MK-6), while the major fatty acids comprised iso-C15:0 (26.7%), 3-OH iso-C17:0 (19.9%), and iso-C15:1 (16.6%). Polar lipids included phosphatidylethanolamine, two unidentified aminolipids, and four unidentified lipids. Genomic annotation identified complete carotenoid biosynthesis pathways (crt-B, crt-I, crt-Z), supporting which may contribute to oxidative stress resistance via β-carotene and zeaxanthin biosynthesis. Based on polyphasic taxonomic evidence encompassing phenotypic, chemotaxonomic, and genomic characteristics, strain CXY002T represents a novel species within the genus Maribacter, for which the name Maribacter luteocoastalis sp. nov. is proposed. The type strain is CXY002T (= MCCC 1K09937T = KCTC 102438T).