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<p>Two Gram-negative-strains, 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> were isolated from tidal flat sediment in Daebudo, Republic of Korea. The isolates are non-motile, and long rods. Both strains are oxidase-positive and grew in the range of 15–45&#xa0;°C (optimum, 40&#xa0;°C) and pH 7–8 (optimum pH 8.0). Growth was observed in the salinity range of 1–6%, with optimum growth at 4.0% for strain 3YJGBD-33<sup>T</sup> and 3.0% for strain 3YJGBD-35<sup>T</sup>. According to the NCBI 16S ribosomal RNA sequences database the strain 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> showed the highest 16S rRNA gene similarity with <i>Robertkochia marina</i> CC-AMO-30D<sup>T</sup> (97.0% and 98.4%), <i>Robertkochia sediminum</i> 1368<sup>T</sup> (96.4% and 98.3%) and <i>Robertkochia solimangrovi</i> CL23<sup>T</sup> (96.0% and 96.5%). The major respiratory quinone is MK-6. The polar lipid profile consists of phosphatidylethanolamine, three aminolipids and two unidentified lipids. The major cellular fatty acids for strains 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> were iso-C<sub>15:0,</sub> iso-C<sub>15:1</sub> G and iso-C<sub>17:0</sub> 3-OH. The draft genome of strains 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> are 3.4&#xa0;Mbp and 3.5&#xa0;Mbp in size with 2993 coding genes, 41 tRNAs and 2935 coding genes, 42 tRNAs, respectively. The polyphasic analysis confirms that strains 3YJGBD-33<sup>T</sup> and 3YJGBD-35<sup>T</sup> belong to the genus <i>Robertkochia</i> and represent novel species, for which the names <i>Robertkochia aurantiaca</i> sp. nov. and <i>Robertkochia flava</i>&#xa0;sp. nov. are proposed. The type strains are 3YJGBD-33<sup>T</sup> (= KCTC 82715<sup>T</sup> = TBRC 19393<sup>T</sup>) and 3YJGBD-35<sup>T</sup> (= KCTC 82716<sup>T</sup> = JCM 34784<sup>T</sup>).</p>

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Robertkochia aurantiaca sp. nov., and Robertkochia flava sp. nov., two potential quinate- and catechol-degrading bacteria isolated from tidal-flat sediments

  • 期刊论文

  • 发表日期：

    2025年7月7日

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  • Jaincy N. Jayan,
  • Young-Mi Lee,
  • Ye-ji You,
  • Sathiyaraj Srinivasan,
  • Sang-Seob Lee

摘要

Two Gram-negative-strains, 3YJGBD-33T and 3YJGBD-35T were isolated from tidal flat sediment in Daebudo, Republic of Korea. The isolates are non-motile, and long rods. Both strains are oxidase-positive and grew in the range of 15–45 °C (optimum, 40 °C) and pH 7–8 (optimum pH 8.0). Growth was observed in the salinity range of 1–6%, with optimum growth at 4.0% for strain 3YJGBD-33T and 3.0% for strain 3YJGBD-35T. According to the NCBI 16S ribosomal RNA sequences database the strain 3YJGBD-33T and 3YJGBD-35T showed the highest 16S rRNA gene similarity with Robertkochia marina CC-AMO-30DT (97.0% and 98.4%), Robertkochia sediminum 1368T (96.4% and 98.3%) and Robertkochia solimangrovi CL23T (96.0% and 96.5%). The major respiratory quinone is MK-6. The polar lipid profile consists of phosphatidylethanolamine, three aminolipids and two unidentified lipids. The major cellular fatty acids for strains 3YJGBD-33T and 3YJGBD-35T were iso-C15:0, iso-C15:1 G and iso-C17:0 3-OH. The draft genome of strains 3YJGBD-33T and 3YJGBD-35T are 3.4 Mbp and 3.5 Mbp in size with 2993 coding genes, 41 tRNAs and 2935 coding genes, 42 tRNAs, respectively. The polyphasic analysis confirms that strains 3YJGBD-33T and 3YJGBD-35T belong to the genus Robertkochia and represent novel species, for which the names Robertkochia aurantiaca sp. nov. and Robertkochia flava sp. nov. are proposed. The type strains are 3YJGBD-33T (= KCTC 82715T = TBRC 19393T) and 3YJGBD-35T (= KCTC 82716T = JCM 34784T).