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<p>An aerobic, Gram-stain-negative bacterium, designated as strain WLY502<sup>T</sup>, was isolated from the environment of <i>Baobaoqu</i> starter production. The strain was with a short rod shape cells 0.5–0.7&#xa0;μm wide and 1.1–2.0&#xa0;μm long, arranged singly or in pairs. The isolate was able to grow at temperatures of 20–37&#xa0;°C (optimum growth at 25&#xa0;°C), at pH 5.0–10.0 (optimum growth at pH 7.0) and in the presence of 0–5% (w/v) NaCl (optimum growth with 0% NaCl). Ribose, xylose, arabinose, mannose, glucose and galactose were the major cell-wall sugar. The <i>meso</i>-diaminopimelic acid (<i>meso</i>-DAP) was the diagnostic amino acid. The main polar lipids of strain WLY502<sup>T</sup> included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), unidentified aminolipids (UAL 1–2), unidentified aminophospholipid (UAPL), unidentified aminophospholipid (UAPL), unidentified phospholipid (UPL), unidentified lipids (UL 1–5), glycolipid (GL). Ubiquinone Q-10 was predominant menaquinone and C<sub>18:1</sub> ω7c (80.5%) was the major fatty acid. Comparisons of 16S rRNA gene sequence indicated that strain WLY502<sup>T</sup> was most closely related to <i>Paracoccus acridae</i> SCU-M53<sup>T</sup> (98.48%), <i>Paracoccus haematequi</i> M1-83<sup>T</sup> (98.40%). The average nucleotide identity (ANI) values strain WLY502<sup>T</sup> and the two type strains mentioned above were 91.19% and 70.96%. The average amino identity (AAI) value between them were 93.42% and 87.19%. Additionally, the digital DNA-DNA hybridization (dDDH) value between them is 42.9% and 30.1%. The genomic DNA G + C content was 64.99%. Strain WLY502<sup>T</sup> could produce astaxanthin and contains several genes (such as MalK, UgpE, UgpA, PRK07067, PBP2 TMBP ike, YcjU, YjiC, crtI-fam, crtY, ERG9, and IspA) involved in astaxanthin. Therefore, strain WLY502<sup>T</sup> may have development and utilisation value in flavouring agents. Based on the evidence presented, strain WLY502<sup>T</sup> (CICC 25237<sup>T</sup> = JCM 36429<sup>T</sup>) is proposed as the type strain of a novel species, designated as <i>Paracoccus yibinensis sp. nov</i>.</p>

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Paracoccus yibinensis sp. nov., a novel bacterium with astaxanthin producing isolated from the environment of Chinese distilled Baijiu

  • 期刊论文

  • 发表日期：

    2025年4月23日

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  • Qingchun Luo,
  • Yanping Lu,
  • Xi Li,
  • Jian Su,
  • Pengju Zhao,
  • Zhu Zhang,
  • Yang Gu,
  • Dong Zhao,
  • Jia Zheng

摘要

An aerobic, Gram-stain-negative bacterium, designated as strain WLY502T, was isolated from the environment of Baobaoqu starter production. The strain was with a short rod shape cells 0.5–0.7 μm wide and 1.1–2.0 μm long, arranged singly or in pairs. The isolate was able to grow at temperatures of 20–37 °C (optimum growth at 25 °C), at pH 5.0–10.0 (optimum growth at pH 7.0) and in the presence of 0–5% (w/v) NaCl (optimum growth with 0% NaCl). Ribose, xylose, arabinose, mannose, glucose and galactose were the major cell-wall sugar. The meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid. The main polar lipids of strain WLY502T included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), unidentified aminolipids (UAL 1–2), unidentified aminophospholipid (UAPL), unidentified aminophospholipid (UAPL), unidentified phospholipid (UPL), unidentified lipids (UL 1–5), glycolipid (GL). Ubiquinone Q-10 was predominant menaquinone and C18:1 ω7c (80.5%) was the major fatty acid. Comparisons of 16S rRNA gene sequence indicated that strain WLY502T was most closely related to Paracoccus acridae SCU-M53T (98.48%), Paracoccus haematequi M1-83T (98.40%). The average nucleotide identity (ANI) values strain WLY502T and the two type strains mentioned above were 91.19% and 70.96%. The average amino identity (AAI) value between them were 93.42% and 87.19%. Additionally, the digital DNA-DNA hybridization (dDDH) value between them is 42.9% and 30.1%. The genomic DNA G + C content was 64.99%. Strain WLY502T could produce astaxanthin and contains several genes (such as MalK, UgpE, UgpA, PRK07067, PBP2 TMBP ike, YcjU, YjiC, crtI-fam, crtY, ERG9, and IspA) involved in astaxanthin. Therefore, strain WLY502T may have development and utilisation value in flavouring agents. Based on the evidence presented, strain WLY502T (CICC 25237T = JCM 36429T) is proposed as the type strain of a novel species, designated as Paracoccus yibinensis sp. nov.