Scholarlink

<p>A Gram-stain-positive, motile, and oval-shaped bacterium, designated as SYSU BS000539<sup>T</sup>, was isolated from black soil in Heilongjiang Province, China. 16S rRNA gene sequencing revealed that the isolate shared the highest similarity to <i>Trujillonella endophytica</i> DSM 45413<sup>T</sup> (98.32%), and phylogenetic analysis confirmed that it belonged to the genus <i>Trujillonella</i>. The strain could grow at temperatures from 15 to 37&#xa0;°C (with an optimum at 28&#xa0;°C), in salt concentrations of 0–5% (w/v) (with an optimum at 0%), and at pH levels from 6.0 to 10.0 (with an optimum at 8.0). The dominant cellular fatty acids (&gt; 5%) were iso-C<sub>16:0</sub>, iso-C<sub>15:0</sub>, and iso-C<sub>16:1</sub>, while the principal respiratory quinones was menaquinone MK-9 (H<sub>4</sub>) with menaquinone MK-8 as minor components. Polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol methylphosphonolipid, an unidentified aminolipid and two unidentified polar lipids. The G + C content of the genome was 74.97%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SYSU BS000539<sup>T</sup> and <i>T. endophytica</i> DSM 45413<sup>T</sup> were 84.10% and 27.60%, respectively, falling below species delineation thresholds. Combining phylogenetic, genotypic, chemotaxonomic, physiological and biochemical analyses, it is evident that SYSU BS000539<sup>T</sup> represented a novel species within the genus <i>Trujillonella</i>, for which the name <i>Trujillonella humicola</i> sp. nov<i>.</i> is proposed. The type strain of the proposed novel species is SYSU BS000539<sup>T</sup> (= MCCC 1K09330<sup>T</sup> = KCTC 59402<sup>T</sup>).</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Trujillonella humicola sp. nov., a siderophore-synthesizing bacterium isolated from black soil in Northeast China

  • 期刊论文

  • 发表日期：

    2025年4月22日

查看全文
  • Ying Wang,
  • Le-Bin Chen,
  • Yu-Ting OuYang,
  • Jia-Ying Xing,
  • Ke Zhang,
  • Nan-Feng Chen,
  • Ting-Ting She,
  • Shuang Wang,
  • Jian-Yu Jiao,
  • Wen-Jun Li

摘要

A Gram-stain-positive, motile, and oval-shaped bacterium, designated as SYSU BS000539T, was isolated from black soil in Heilongjiang Province, China. 16S rRNA gene sequencing revealed that the isolate shared the highest similarity to Trujillonella endophytica DSM 45413T (98.32%), and phylogenetic analysis confirmed that it belonged to the genus Trujillonella. The strain could grow at temperatures from 15 to 37 °C (with an optimum at 28 °C), in salt concentrations of 0–5% (w/v) (with an optimum at 0%), and at pH levels from 6.0 to 10.0 (with an optimum at 8.0). The dominant cellular fatty acids (> 5%) were iso-C16:0, iso-C15:0, and iso-C16:1, while the principal respiratory quinones was menaquinone MK-9 (H4) with menaquinone MK-8 as minor components. Polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol methylphosphonolipid, an unidentified aminolipid and two unidentified polar lipids. The G + C content of the genome was 74.97%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SYSU BS000539T and T. endophytica DSM 45413T were 84.10% and 27.60%, respectively, falling below species delineation thresholds. Combining phylogenetic, genotypic, chemotaxonomic, physiological and biochemical analyses, it is evident that SYSU BS000539T represented a novel species within the genus Trujillonella, for which the name Trujillonella humicola sp. nov. is proposed. The type strain of the proposed novel species is SYSU BS000539T (= MCCC 1K09330T = KCTC 59402T).