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<p>A novel endophytic actinomycete with antagonistic activity against <i>Fusarium moniliforme</i>, designated strain NEAU-BLH26<sup>T</sup>, was isolated from the root of <i>Adonis amurensis</i> Regel collected at the horticultural experiment station of Northeast Agricultural University, Heilongjiang Province, northeast China. Strain NEAU-BLH26<sup>T</sup> exhibited morphological and chemotaxonomic features of the genus <i>Streptomyces</i>. The diamino acid present in its cell wall was identified as LL-diaminopimelic acid, while galactose detected in whole-cell hydrolysates. The menaquinones were identified as MK-9(H<sub>6</sub>), MK-9(H<sub>8</sub>), and MK-9(H<sub>4</sub>). The phospholipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified aminolipids. The major fatty acids were determined to be <i>iso</i>-C<sub>16:0</sub> and C<sub>16:0</sub>. The DNA G + C content, based on the genome sequence, was 70.5&#xa0;mol%. Phylogenetic analyses of 16S rRNA gene and whole genome sequences analyses indicated that strain NEAU-BLH26<sup>T</sup> was most closely related to <i>Streptomyces geranii</i> A301<sup>T</sup>, with a 16S rRNA gene sequence similarity of 98.69%. However, the average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values confirmed that strain NEAU-BLH26<sup>T</sup> represents a distinct species. This conclusion was further supported by phenotypic and chemotaxonomic differences between strain NEAU-BLH26<sup>T</sup> and its closest relative. Additionally, the secondary metabolite biosynthetic gene clusters in strain NEAU-BLH26<sup>T</sup> were predicted and analyzed. Based on phenotypic, chemotaxonomic and genotypic evidence, strain NEAU-BLH26<sup>T</sup> is proposed as a novel species of the genus <i>Streptomyces</i>, for which the name <i>Streptomyces adonidis</i> sp. nov. is suggested. The type strain is NEAU-BLH26<sup>T</sup> (= JCM 36414<sup>T</sup> = MCCC 1K08678<sup>T</sup>).</p>

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Streptomyces adonidis sp. nov., a novel actinomycete strain with antifungal activity isolated from the root of Adonis amurensis Regel

  • 期刊论文

  • 发表日期：

    2025年3月25日

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  • Rui Du,
  • Hui Bing,
  • Wentian Lu,
  • Congting Gao,
  • Fanxue Kong,
  • Hongtu Wang,
  • Yue Shen,
  • Chongxi Liu,
  • Wensheng Xiang

摘要

A novel endophytic actinomycete with antagonistic activity against Fusarium moniliforme, designated strain NEAU-BLH26T, was isolated from the root of Adonis amurensis Regel collected at the horticultural experiment station of Northeast Agricultural University, Heilongjiang Province, northeast China. Strain NEAU-BLH26T exhibited morphological and chemotaxonomic features of the genus Streptomyces. The diamino acid present in its cell wall was identified as LL-diaminopimelic acid, while galactose detected in whole-cell hydrolysates. The menaquinones were identified as MK-9(H6), MK-9(H8), and MK-9(H4). The phospholipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified aminolipids. The major fatty acids were determined to be iso-C16:0 and C16:0. The DNA G + C content, based on the genome sequence, was 70.5 mol%. Phylogenetic analyses of 16S rRNA gene and whole genome sequences analyses indicated that strain NEAU-BLH26T was most closely related to Streptomyces geranii A301T, with a 16S rRNA gene sequence similarity of 98.69%. However, the average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values confirmed that strain NEAU-BLH26T represents a distinct species. This conclusion was further supported by phenotypic and chemotaxonomic differences between strain NEAU-BLH26T and its closest relative. Additionally, the secondary metabolite biosynthetic gene clusters in strain NEAU-BLH26T were predicted and analyzed. Based on phenotypic, chemotaxonomic and genotypic evidence, strain NEAU-BLH26T is proposed as a novel species of the genus Streptomyces, for which the name Streptomyces adonidis sp. nov. is suggested. The type strain is NEAU-BLH26T (= JCM 36414T = MCCC 1K08678T).