<p>Semi-natural areas support a variety of pollinators, which can enhance pollination and increase the yields of many crops. This study evaluates the influence of both pollination and forest-stand configuration on the seed dry mass and fatty acid composition of soybean. At each site, plots with pollinator-excluded and pollinator-exposed plants were established at different distances from the forest. We collected flowers (to quantify pollen grains on stigma) and seeds from these treatments across ten soybean stands. The amount of potentially suitable habitat for pollinators (forests and semi-natural areas) was quantified around each of the sampling sites. Pollination had a positive effect on all variables. Specifically, pollinator-exposed plants had 93% higher pollen deposition, 31% heavier dry seed mass, 6% more seed oil content and a 6.8% greater oleic acid concentration. Furthermore, most response variables in pollinator-exposed plants increased as the proportion of forest surrounding the crop stands increased. Conversely, only seed mass decreased with increasing distance from the forest edge. Despite soybeans being autogamous, our results highlight that native forests act as a source of pollinators and pollination services for soybean, thereby improving yields. The larger the forested area surrounding soybean stands, the greater the benefits of pollination on yield and seed-oil profiles; however, these positive effects at the forest-stand level can diminish with increasing distance of soybean plants from the forest edge. Overall, these results underscore the critical importance of conservation policies for the remaining forests in agro-ecosystems to enhance pollination and to increase yields in this and other pollinator-dependent crops.</p>