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<p><i>Serratia marcescens</i> is widely used as a model strain in aerobiological researches for its apparent colony morphology and easy removablilty from the working environment. In contrast, <i>Bacillus subtilis</i> var. <i>niger</i> ATCC 9372 is highly resistant to environmental stresses, so requires a laboring decontamination process after test. To substitute for this traditional test strain in the biological testing of Class II Biosafety Cabinets (BSC), we evaluated the characteristics of <i>S. marcescens</i> bioaerosol including count median diameter (CMD), the ratio of single cells, and the total viable CFU (Colony forming unit) stability. When the suspension of the <i>S. marcescens</i> for bioaerosol production was prepared via shake culture, the CMD of <i>S. marcescens</i> bioaerosol was 1.72&#xa0;μm, the ratio of single cells was 92.6%, in accordance with the NSF/ANSI 49 standards. However, the total viable CFU of the <i>S. marcescens</i> bioaerosol tended to decline when prolonged storage of the primary slant culture and the suspension. <i>S. marcescens</i> bioaerosol might be used as a test strain for the biological testing of BSC only when the suspension is prepared using shake culture with a predetermined storage duration of the primary slant culture and the suspension. The relative accuracy, relative specificity, and relative sensitivity of this alternative method using <i>S. marcescens</i> bioaerosol was 92.6%, 91.7%, and 93.3% in personnel protection, 92.6%, 87.5%, and 94.7% in product protection of the biological testing of BSC when compared with the standard method using <i>B. subtilis</i> var. <i>niger</i>.</p>

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Assessment of Serratia marcescens bioaerosol for the biological testing of biosafety cabinet

  • 期刊论文

  • 发表日期：

    2026年1月12日

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  • Ji-Yon Kye,
  • Hyon-Hak Pak,
  • Jong-Hyok Jon,
  • Hyok-Chol Choe,
  • Jong-Sim Ri,
  • Song-Nam Hong,
  • Su-Ryon Ryom

摘要

Serratia marcescens is widely used as a model strain in aerobiological researches for its apparent colony morphology and easy removablilty from the working environment. In contrast, Bacillus subtilis var. niger ATCC 9372 is highly resistant to environmental stresses, so requires a laboring decontamination process after test. To substitute for this traditional test strain in the biological testing of Class II Biosafety Cabinets (BSC), we evaluated the characteristics of S. marcescens bioaerosol including count median diameter (CMD), the ratio of single cells, and the total viable CFU (Colony forming unit) stability. When the suspension of the S. marcescens for bioaerosol production was prepared via shake culture, the CMD of S. marcescens bioaerosol was 1.72 μm, the ratio of single cells was 92.6%, in accordance with the NSF/ANSI 49 standards. However, the total viable CFU of the S. marcescens bioaerosol tended to decline when prolonged storage of the primary slant culture and the suspension. S. marcescens bioaerosol might be used as a test strain for the biological testing of BSC only when the suspension is prepared using shake culture with a predetermined storage duration of the primary slant culture and the suspension. The relative accuracy, relative specificity, and relative sensitivity of this alternative method using S. marcescens bioaerosol was 92.6%, 91.7%, and 93.3% in personnel protection, 92.6%, 87.5%, and 94.7% in product protection of the biological testing of BSC when compared with the standard method using B. subtilis var. niger.