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Background <p>Exposure to airborne dust particles increases&#xa0;the risks of respiratory illnesses, including asthma, chronic obstructive pulmonary disease, and bronchitis. Determining effective interventional strategies to mitigate toxicity induced by airborne dust particles may provide essential public health benefits. This study assessed the detrimental effects of airborne dust particles extracted from car air filters in Lahore City, alongside the therapeutic potential of adipose-derived stem cell conditioned medium (ADSC-CM) on cabin air filter (CAF) dust-induced lung damage in mice.</p> Methods <p>BALB/c mice were subjected to repeated intraperitoneal sensitization with CAF dust suspension followed by challenges with aerosolized dust in a nebulization chamber. Moreover, the treated group of mice received intravenous injections of ADSCs-CM before exposure to CAFs dust challenges. Subsequently, allergic manifestations, lung histopathological features, synthesis of inflammatory cytokines, and potential gene expressions linked to pulmonary diseases were investigated.</p> Results <p>CAF dust exposure triggered lung injury and inflammation characterized by epithelial sloughing, hyperplasia, pulmonary edema, and infiltration of inflammatory cells into the bronchi. Further studies showed an&#xa0;increased synthesis of inflammatory cytokines, notably IL-6 and TNF-α, and elevated expression of COX-2 and MUC5AC gene. Supplementation with ADSCs-CM substantially ameliorated lung inflammation, reduced allergic symptoms, induction of cytokines, COX-2, and MUC5AC genes expression.</p> Conclusion <p>CAF dust samples showed harmful effects on lung health, depicting potential risks to city residents. The results exhibited that ADSCs-CM transplantation substantially reduced lung inflammation and could serve as a promising treatment for pulmonary disorders.</p>

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Airborne dust particle-induced lung inflammation in mice alleviated by adipose stem cell-derived conditioned medium

  • 期刊论文

  • 发表日期：

    2026年1月7日

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  • Hazrat Zaman,
  • Madiha Habib,
  • Zobia Safdar,
  • Shafi Zaman,
  • Hafiza Sumbul Yousaf,
  • Muhammad Shahbaz Aslam,
  • Zaigham Abbas

摘要

Background

Exposure to airborne dust particles increases the risks of respiratory illnesses, including asthma, chronic obstructive pulmonary disease, and bronchitis. Determining effective interventional strategies to mitigate toxicity induced by airborne dust particles may provide essential public health benefits. This study assessed the detrimental effects of airborne dust particles extracted from car air filters in Lahore City, alongside the therapeutic potential of adipose-derived stem cell conditioned medium (ADSC-CM) on cabin air filter (CAF) dust-induced lung damage in mice.

Methods

BALB/c mice were subjected to repeated intraperitoneal sensitization with CAF dust suspension followed by challenges with aerosolized dust in a nebulization chamber. Moreover, the treated group of mice received intravenous injections of ADSCs-CM before exposure to CAFs dust challenges. Subsequently, allergic manifestations, lung histopathological features, synthesis of inflammatory cytokines, and potential gene expressions linked to pulmonary diseases were investigated.

Results

CAF dust exposure triggered lung injury and inflammation characterized by epithelial sloughing, hyperplasia, pulmonary edema, and infiltration of inflammatory cells into the bronchi. Further studies showed an increased synthesis of inflammatory cytokines, notably IL-6 and TNF-α, and elevated expression of COX-2 and MUC5AC gene. Supplementation with ADSCs-CM substantially ameliorated lung inflammation, reduced allergic symptoms, induction of cytokines, COX-2, and MUC5AC genes expression.

Conclusion

CAF dust samples showed harmful effects on lung health, depicting potential risks to city residents. The results exhibited that ADSCs-CM transplantation substantially reduced lung inflammation and could serve as a promising treatment for pulmonary disorders.