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<p>Fin amputation in fish is one of the procedures that is routinely performed in fisheries research, such as for genomic screening or studying fish populations. Unfortunately, this procedure is painful for fish and could adversely affect a variety of their behaviors. However, there were still big gaps in the knowledge of behavioral response differences between various fish species after a caudal fin excision. Therefore, the present study aimed to address and comprehensively compare the impact of acute stress induced by a caudal fin amputation on the modulation of behavioral responses of eight fish species, which were <i>Chromobotia macracanthus</i>, <i>Balantiocheilos melanopterus</i>, <i>Acarichthys heckelii</i>, <i>Puntigrus tetrazona</i>, <i>Amatitlania nigrofasciata</i>, <i>Oryzias latipes</i>, <i>Oryzias woworae</i>, and <i>Danio rerio</i>. Behavior was assessed at baseline (pre-amputation), and at 1&#xa0;h, 1&#xa0;day, 3&#xa0;days, and 5&#xa0;days post-amputation (1&#xa0;hpa, 1&#xa0;dpa, 3&#xa0;dpa, and 5&#xa0;dpa, respectively). From the results, a trend of a decrease in locomotion activity was shown in the majority of the tested fish in different magnitudes, with a gradual recovery from 3 or 5&#xa0;dpa. However, <i>C. macracanthus</i>, <i>B. melanopterus</i>, and <i>A. heckelii</i> displayed a relatively unchanged locomotor activity at 1&#xa0;hpa, with a trend of a decrement that occurred at a later observation time for <i>C. macracanthus</i> and <i>A. heckelii</i>. Later, this result was also supported by the PCA and heatmap clustering analyses that grouped these three fishes in a single major cluster since these fishes displayed a different acute behavior response, especially in terms of exploratory behaviors, than other tested fishes, including zebrafish (<i>D. rerio</i>). Taken together, this study highlighted that acute stress by caudal fin amputation might not typically result in profound behavior changes in some fish species. In addition, the current findings also might contribute to improving the current knowledge of nociceptive responses in various species of fish that could help in fish refinement that could be adopted across a wide range of biological disciplines.</p>

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Comprehensive comparisons of behavior performances in various fish species after caudal fin amputation by using a 3D locomotion test

  • 期刊论文

  • 发表日期：

    2026年3月19日

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  • Gilbert Audira,
  • Tzu-Ming Tseng,
  • Kevin Adi Kurnia,
  • Chih-Hsin Hung,
  • Chung-Der Hsiao,
  • Cheng-Yo Yen

摘要

Fin amputation in fish is one of the procedures that is routinely performed in fisheries research, such as for genomic screening or studying fish populations. Unfortunately, this procedure is painful for fish and could adversely affect a variety of their behaviors. However, there were still big gaps in the knowledge of behavioral response differences between various fish species after a caudal fin excision. Therefore, the present study aimed to address and comprehensively compare the impact of acute stress induced by a caudal fin amputation on the modulation of behavioral responses of eight fish species, which were Chromobotia macracanthus, Balantiocheilos melanopterus, Acarichthys heckelii, Puntigrus tetrazona, Amatitlania nigrofasciata, Oryzias latipes, Oryzias woworae, and Danio rerio. Behavior was assessed at baseline (pre-amputation), and at 1 h, 1 day, 3 days, and 5 days post-amputation (1 hpa, 1 dpa, 3 dpa, and 5 dpa, respectively). From the results, a trend of a decrease in locomotion activity was shown in the majority of the tested fish in different magnitudes, with a gradual recovery from 3 or 5 dpa. However, C. macracanthus, B. melanopterus, and A. heckelii displayed a relatively unchanged locomotor activity at 1 hpa, with a trend of a decrement that occurred at a later observation time for C. macracanthus and A. heckelii. Later, this result was also supported by the PCA and heatmap clustering analyses that grouped these three fishes in a single major cluster since these fishes displayed a different acute behavior response, especially in terms of exploratory behaviors, than other tested fishes, including zebrafish (D. rerio). Taken together, this study highlighted that acute stress by caudal fin amputation might not typically result in profound behavior changes in some fish species. In addition, the current findings also might contribute to improving the current knowledge of nociceptive responses in various species of fish that could help in fish refinement that could be adopted across a wide range of biological disciplines.