<p>Abiotic stress corresponds to climatic variables which inhibit plant growth as well as development. It became the attention of plant scientists and breeders as yield of rice crop loss due to various abiotic stresses. Plant stress response mechanism is controlled by the alpha-ketoglutarate-dependent dioxygenase, AlkB homolog (ALKBH) gene family. Seven ALKBH genes were identified in rice based on phylogenetic analysis. In silico investigations were carried out in the current work to determine the crucial function of ALKBH gene family in abiotic conditions. Physical characteristics, gene structure, motif composition, phylogenetic analysis, chromosome distribution, cis-acting element prediction, and synteny analysis were among the standardized analyses that were carried out. The subcellular expression of <i>OsALKBH</i> was found in the cytoplasm and nucleus. According to biological data analysis, we identified gene structure and motif distribution, intron, exon number, motif arrangement molecular weight and their amino acid lengths of ALKBH proteins. The ALKBH genes showed different responses to abiotic stresses and dynamic spatiotemporal expression patterns. Its exon/intron sequences and protein motifs are highly conserved. We identified the function of ALKBH gene in rice under abiotic stresses The present study was conducted to investigate this gene family in rice (<i>Oryza sativa</i>) by using in silico analysis, to investigate the possible function of its members in abiotic conditions. Results of gene expression in the rice sub genome were revealed by RNA-Seq, suggesting their function under abiotic stress conditions particularly and in plant growth. The results of this study demonstrated its potential for future use with aimed at exploring functions of ALKBH genes for developing stress-tolerance in rice.</p>