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<p>Major bacterial diseases of potato, such as bacterial wilt, soft rot, and blackleg, cause significant tuber losses. Controlling potato diseases mainly relies on pesticides, which harm the environment. Biological control, which utilizes beneficial microbes to suppress pathogens and enhance plant resistance, provides a&#xa0;safer alternative. In this study, nine <i>Streptomyces</i> spp. isolates were obtained from potato rhizosphere soils, among which isolates BIR3 and ABU8 exhibited strong antibacterial activity against three major potato pathogens. These isolates effectively inhibited the growth of <i>Ralstonia solanacearum, Pectobacterium carotovorum</i>, and <i>Pectobacterium atrosepticum in vitro</i>. Morphological, cultural, and molecular analyses identified both isolates as <i>Streptomyces rochei</i>. <i>In planta, S</i>.&#xa0;<i>rochei</i> ABU8 reduced the disease index of bacterial wilt in tomato to 25.6%, while BIR3 reduced it to 36.8%, compared to 86.4% in untreated plants. <i>S</i>.&#xa0;<i>rochei</i> BIR3 achieved 100% protection against soft rot of potato caused by <i>P</i>. <i>atrosepticum</i> and 73.3% against soft rot of potato caused by <i>P</i>. <i>carotovorum</i>, while ABU8 showed 93.9% and 58.4% protection, respectively. The ethyl acetate (EtOAc) extract of <i>S</i>.&#xa0;<i>rochei</i> ABU8 exhibited antibacterial activity against the tested pathogens. GC-MS analysis of the extract revealed the presence of several bioactive compounds, including 2.3-butanediol, lactic acid, pyrrole-2-carboxylic acid, cyclo(glycylprolyl), cyclo(leucylprolyl), and palmitic acid, which are likely to contribute to the observed antibacterial activity. These results suggest that <i>S</i>. <i>rochei</i> is a&#xa0;promising biocontrol agent for managing bacterial potato diseases, offering a&#xa0;sustainable alternative to chemical treatments.</p>

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Evaluation of Streptomyces rochei Strains as Potential Biocontrol Agents Against Key Bacterial Pathogens of Potato

  • 期刊论文

  • 发表日期：

    2026年3月3日

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  • Ashraf Fathy Abd El-Rahman,
  • Omnia Ahmed Abd El-Hafez,
  • El-Shaimaa Mostafa Abd El-Hamed

摘要

Major bacterial diseases of potato, such as bacterial wilt, soft rot, and blackleg, cause significant tuber losses. Controlling potato diseases mainly relies on pesticides, which harm the environment. Biological control, which utilizes beneficial microbes to suppress pathogens and enhance plant resistance, provides a safer alternative. In this study, nine Streptomyces spp. isolates were obtained from potato rhizosphere soils, among which isolates BIR3 and ABU8 exhibited strong antibacterial activity against three major potato pathogens. These isolates effectively inhibited the growth of Ralstonia solanacearum, Pectobacterium carotovorum, and Pectobacterium atrosepticum in vitro. Morphological, cultural, and molecular analyses identified both isolates as Streptomyces rochei. In planta, S. rochei ABU8 reduced the disease index of bacterial wilt in tomato to 25.6%, while BIR3 reduced it to 36.8%, compared to 86.4% in untreated plants. S. rochei BIR3 achieved 100% protection against soft rot of potato caused by P. atrosepticum and 73.3% against soft rot of potato caused by P. carotovorum, while ABU8 showed 93.9% and 58.4% protection, respectively. The ethyl acetate (EtOAc) extract of S. rochei ABU8 exhibited antibacterial activity against the tested pathogens. GC-MS analysis of the extract revealed the presence of several bioactive compounds, including 2.3-butanediol, lactic acid, pyrrole-2-carboxylic acid, cyclo(glycylprolyl), cyclo(leucylprolyl), and palmitic acid, which are likely to contribute to the observed antibacterial activity. These results suggest that S. rochei is a promising biocontrol agent for managing bacterial potato diseases, offering a sustainable alternative to chemical treatments.