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<p><i>Pinus massoniana</i> is a pioneer and light-demanding tree species. The ecological differences between gap and understory environments are influenced primarily by factors such as light, humidity, and plant growth strategies. However, the transcriptional mechanisms underlying the responses of <i>P. massoniana</i> seedlings to environmental variations remain unclear. In this study, <i>P. massoniana</i> seedlings were used as experimental materials, and changes in the leaf transcriptome and related indices were analyzed under different forest ages and light conditions (gap and understory) through underforest planting monitoring. The results showed that both the seed germination rate and the net seedling growth rate of <i>P. massoniana</i> were lower in the understory than in the gap. There were no significant differences in net seedling growth rates among the different forest ages (<i>p</i> &gt; 0.05). However, the seed germination rate and survival rate were higher in mature forests compared to other forest ages. Transcriptome sequencing generated 48,971,721 clean reads. KEGG and GO enrichment analyses revealed that flavonoid biosynthesis and systemic acquired resistance mediated by salicylic acid were the core pathways involved in adaptation to heterogeneous conditions between gaps and understories. Furthermore, fatty acid-binding protein 3 (FAP3), chloroplastic 3, putative anthocyanidin reductase (ANR), and leucoanthocyanidin reductase (LAR) were identified as key regulatory genes in the flavonoid metabolic pathway. These findings provide new insights into the adaptation strategies of <i>P. massoniana</i> seedlings in natural forest environments.</p>

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Transcriptomic analysis of Pinus massoniana seedlings in response to the differential mechanisms of canopy gaps and understory environments

  • 期刊论文

  • 发表日期：

    2026年1月20日

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  • Wenxuan Quan,
  • Qiyu Li,
  • Chao Zhao,
  • Chaochan Li,
  • Guijie Ding

摘要

Pinus massoniana is a pioneer and light-demanding tree species. The ecological differences between gap and understory environments are influenced primarily by factors such as light, humidity, and plant growth strategies. However, the transcriptional mechanisms underlying the responses of P. massoniana seedlings to environmental variations remain unclear. In this study, P. massoniana seedlings were used as experimental materials, and changes in the leaf transcriptome and related indices were analyzed under different forest ages and light conditions (gap and understory) through underforest planting monitoring. The results showed that both the seed germination rate and the net seedling growth rate of P. massoniana were lower in the understory than in the gap. There were no significant differences in net seedling growth rates among the different forest ages (p > 0.05). However, the seed germination rate and survival rate were higher in mature forests compared to other forest ages. Transcriptome sequencing generated 48,971,721 clean reads. KEGG and GO enrichment analyses revealed that flavonoid biosynthesis and systemic acquired resistance mediated by salicylic acid were the core pathways involved in adaptation to heterogeneous conditions between gaps and understories. Furthermore, fatty acid-binding protein 3 (FAP3), chloroplastic 3, putative anthocyanidin reductase (ANR), and leucoanthocyanidin reductase (LAR) were identified as key regulatory genes in the flavonoid metabolic pathway. These findings provide new insights into the adaptation strategies of P. massoniana seedlings in natural forest environments.