Scholarlink

<p>Healing of stem bark wounds is important for minimizing pathogen infection risk, restoring nutrient transport and structural support in trees. Here, we explore how propagation through somatic embryogenesis (SE) and methyl jasmonate (MeJA) treatment affect wound healing ability in Norway spruce (<i>Picea abies</i>) plants. We inflicted a mechanical wound on the lower stem of MeJA- and non-treated plants produced via SE (emblings) or from seeds (seedlings). Visible signs of healing around the wound edges (onset of healing) were recorded 2 weeks post-wounding; wound size (exposed xylem) was measured every other week (June–September) in year 1, and May and September in year 2. Plant height and diameter were also measured. MeJA positively affected healing onset, with 48% more MeJA- than non-treated plants exhibiting early healing. This resulted in a sharp decrease in wound size for MeJA-treated plants 2–4 weeks post-wounding. However, these benefits only occurred early on, as MeJA reduced the overall healing rate (tissue growth/day) by 9%. For SE, fewer emblings (70%) showed early healing signs compared to seedlings (91%). Yet, non-treated emblings showed the highest healing rate during year 1; in year 2, these effects persisted with all emblings having a 61% faster healing rate and 68% more had completely closed their wounds relative to seedlings. Wounding did not affect growth, MeJA negatively affected diameter but not height, and overall emblings grew less than seedlings. We conclude that MeJA may stimulate stem wound healing initiation in Norway spruce, but slow down healing rate and vice versa for SE plants.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effects of propagation method and methyl jasmonate treatment on stem bark wound healing in Norway spruce seedlings

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年6月27日

查看全文
  • Kristina Berggren,
  • Amelia Tudoran,
  • Yayuan Chen,
  • Mikko Tikkinen,
  • Helena Bylund,
  • Christer Björkman,
  • Ulrika Egertsdotter,
  • Adriana Puentes

摘要

Healing of stem bark wounds is important for minimizing pathogen infection risk, restoring nutrient transport and structural support in trees. Here, we explore how propagation through somatic embryogenesis (SE) and methyl jasmonate (MeJA) treatment affect wound healing ability in Norway spruce (Picea abies) plants. We inflicted a mechanical wound on the lower stem of MeJA- and non-treated plants produced via SE (emblings) or from seeds (seedlings). Visible signs of healing around the wound edges (onset of healing) were recorded 2 weeks post-wounding; wound size (exposed xylem) was measured every other week (June–September) in year 1, and May and September in year 2. Plant height and diameter were also measured. MeJA positively affected healing onset, with 48% more MeJA- than non-treated plants exhibiting early healing. This resulted in a sharp decrease in wound size for MeJA-treated plants 2–4 weeks post-wounding. However, these benefits only occurred early on, as MeJA reduced the overall healing rate (tissue growth/day) by 9%. For SE, fewer emblings (70%) showed early healing signs compared to seedlings (91%). Yet, non-treated emblings showed the highest healing rate during year 1; in year 2, these effects persisted with all emblings having a 61% faster healing rate and 68% more had completely closed their wounds relative to seedlings. Wounding did not affect growth, MeJA negatively affected diameter but not height, and overall emblings grew less than seedlings. We conclude that MeJA may stimulate stem wound healing initiation in Norway spruce, but slow down healing rate and vice versa for SE plants.