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<p>Conservation and assessment of germplasm resources are vital for forest genetics and breeding. <i>Eucalyptus cloeziana</i> F. Muell is a valuable timber tree speciesthat is prized for its substantial economic and ecological importance. Nevertheless, research on its functional genomics and molecular breeding remains limited. In this study, 20 fluorescently-labeled polymorphic EST-SSR markers were utilized to genotype 448 <i>E. cloeziana</i> individuals from 118 families originating from 19 provenances at the State-owned Dongmen Forest Farm in Guangxi, China, one of the largest gene pools of <i>Eucalyptus</i> in Asia. The results unveiled a relatively high genetic diversity in <i>E. cloeziana</i> germplasm (<i>I</i> = 2.310; <i>He</i> = 0.855) and low genetic differentiation among provenances, with 96.73% genetic variation occurring within provenances. Bayesian structure analysis grouped the tested accessions into three clusters: northern provenances, northern high-altitude provenances, and southern provenances. A core collection comprising 85 individuals (18.97% of the total breeding population of 448 individuals) was established through random non-repetitive sampling to minimize redundancy while preserving a high level of genetic diversity (<i>I</i> = 2.470; <i>He</i> = 0.890). Three environment-associated loci (EC-e039, EC-e015, and EC-e089) were identified via a latent factor mixed model (LFMM) and redundancy analysis (RDA). Two temperature variables (maximum temperature of the warmest month and mean temperature of the driest quarter) and two precipitation variables (annual precipitation and precipitation of the driest month) were identified as significant environmental factors influencing adaptive variation in the species. These findings highlight the close relationship between climate conditions and genetic variability, providing valuable insights for managing forest species in a rapidly changing environment.</p>

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Genetic diversity, core collection construction and genotype–environment associations of Eucalyptus cloeziana germplasm based on EST-SSR markers

  • 期刊论文

  • 发表日期：

    2025年4月19日

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  • Hui Lu,
  • Xinyuan Liang,
  • Jianzhong Wang,
  • Tao Xiong,
  • Zeng-Fu Xu,
  • Tiandao Bai,
  • Weixin Jiang

摘要

Conservation and assessment of germplasm resources are vital for forest genetics and breeding. Eucalyptus cloeziana F. Muell is a valuable timber tree speciesthat is prized for its substantial economic and ecological importance. Nevertheless, research on its functional genomics and molecular breeding remains limited. In this study, 20 fluorescently-labeled polymorphic EST-SSR markers were utilized to genotype 448 E. cloeziana individuals from 118 families originating from 19 provenances at the State-owned Dongmen Forest Farm in Guangxi, China, one of the largest gene pools of Eucalyptus in Asia. The results unveiled a relatively high genetic diversity in E. cloeziana germplasm (I = 2.310; He = 0.855) and low genetic differentiation among provenances, with 96.73% genetic variation occurring within provenances. Bayesian structure analysis grouped the tested accessions into three clusters: northern provenances, northern high-altitude provenances, and southern provenances. A core collection comprising 85 individuals (18.97% of the total breeding population of 448 individuals) was established through random non-repetitive sampling to minimize redundancy while preserving a high level of genetic diversity (I = 2.470; He = 0.890). Three environment-associated loci (EC-e039, EC-e015, and EC-e089) were identified via a latent factor mixed model (LFMM) and redundancy analysis (RDA). Two temperature variables (maximum temperature of the warmest month and mean temperature of the driest quarter) and two precipitation variables (annual precipitation and precipitation of the driest month) were identified as significant environmental factors influencing adaptive variation in the species. These findings highlight the close relationship between climate conditions and genetic variability, providing valuable insights for managing forest species in a rapidly changing environment.