<p>Minerals play a&#xa0;vital role in plant cell function, serving as structural components, co-factors in enzyme activation, catalysts in metabolism, regulators of osmotic colloids, and facilitators in nutrient and energy transfer. However, an excess or a&#xa0;lack of availability of these minerals in the plant tissue may lead to several maladies, especially physiological disorders. Mango, an economically important crop, faces several bottlenecks in production and postharvest quality caused by mineral imbalances. Due to the lack of a clear understanding of jelly-seed disorder genesis and the limited research on this topic, a&#xa0;study was carried out to investigate the physiological and biochemical basis of jelly seed disorder incidence in ‘Langra’ and ‘Dashehari’ mango varieties in relation to mineral nutrition. Mango varieties were analyzed for major and minor minerals, with their pulp divided into three types,&#xa0;i.e., affected pulp (AP), pulp surrounding affected pulp (PSAP), and healthy pulp (HP). The results revealed that the mineral constituents of jelly seed-affected fruit such as&#xa0;N (0.67 and 0.64%), P (0.12 and 0.14%), and Mg (29.57 and 37.52 mg 100 g⁻¹) were higher, while Ca (11.46 and 11.80 mg 100 g⁻¹), K (0.09 and 0.08%), Zn (0.78 and 0.87 mg 100 g⁻¹), and Cu (0.39 and 0.33 mg 100 g⁻¹) were lower in the ‘Langra’ and Dashehari’ varieties, respectively. The significant mineral imbalance led to the jelly seed incidence. The in-depth understanding on jelly seed disorder provided by the study enables the scientific community to further establish management strategies for the disorder.</p> Graphic abstract <p></p>