<p>Reducing atmospheric carbon dioxide (CO₂) is critical in climate change prevention. Carbon sequestration is considered to be an important ecosystem process that can be used for this purpose. Fruit trees, which have high carbon storage capacity, can serve as carbon sinks. This study aimed to determine the carbon sequestration potential of fruit trees grown in urban and agricultural areas. To this end, the trunk, branch, leaf, and root biomass as well as the soil organic carbon stocks of fruit trees located in different spatial areas were evaluated. Carbon sequestration capacity was compared according to species, age, and land use type. Allometric equations were used to estimate biomass. The findings showed that the carbon sequestration potential of fruit trees can vary across urban and agricultural areas. This potential depends on tree species, age, and management practices. The results show that fruit trees play an important role not only in agricultural production but also in climate change prevention. Considering the carbon sequestration potential of fruit trees in urban infrastructure planning can contribute to environmental sustainability. The findings obtained under Niğde conditions are comparable to fruit tree carbon sequestration estimates reported for other semi-arid regions in the Mediterranean and Central Asia, demonstrating that the proposed framework has a&#xa0;wider range of applications.</p>