Scholarlink

<p>Walnuts hold significant importance due to their economical and nutritional value. However, walnuts possess challenges in <i>in vitro</i> establishment of cultures due to their recalcitrant nature. Optimizing explants, sterilization procedures, nutrient medium, and plant growth regulators, are integral parts for developing a&#xa0;successful micropropagation protocol. To carry out this research, different explants viz., vegetative buds (shoot tips and nodal segments, axillary buds and lateral flushes), meristems, leaf discs, and petioles were selected. Among vegetative buds, axillary buds were found to be the most effective explants for <i>in vitro</i> establishment and shoot initiation with a&#xa0;highest survival rate of 36.33 ± 0.19%, while lateral flushes were found to be more susceptible to browning and contamination and showed the highest survival rate of 09.55 ± 0.29%. Different nutrient media viz., MS (Murashige and Skoog), WPM (Woody Plant Medium), DKWc (Driver and Kuniyuki medium containing only FeEDTA), DKWm (medium containing half concentrations of both FeEDTA and FeEDDHA), and DKWf (medium containing only FeEDDHA) were tested for the establishment of vegetative buds, but DKW medium was found to be most effective in inducing shoot initiation with an average survival rate of 54.99 ± 0.33%. However, phenolic exudation and explant browning were also maximum in DKWc (49.77 ± 0.29%) and lowest in WPM medium (9.66 ± 0.11%). Explant necrosis was observed to be highest in the MS medium (74.88 ± 0.39%) followed by WPM (55.33 ± 0.50%). As DKWf medium gives the highest survival percentage with a&#xa0;high explant browning rate, the DKWf medium was tested with the addition of different antioxidants. It was found that a&#xa0;combination of PVP, activated charcoal, and phloroglucinol was the most effective, reducing explant browning from 25.00 ± 0.19% to 19.77 ± 0.29%. Apart from vegetative buds, meristems were also found to be the least susceptible with the highest survival percentage, but successful conversion to plantlets was not achieved. <i>In vitro</i> establishment of walnut is a&#xa0;complicated process, not least due to intricate physiological and biochemical properties, which include dormancy, slow growth rate, and sensitivity to tissue culture medium. Thus, this ongoing exploration highlights the complexity of walnut tissue culture establishment process and underscores the need for further research to improve the success rate of propagation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Overcoming Challenges in Walnut (Juglans Regia L.) Micropropagation: A Comprehensive Approach to Optimizing Explant Selection, Sterilization, and Nutrient Media

  • 期刊论文

  • 发表日期：

    2025年1月3日

查看全文
  • Sidharth Sharma,
  • Shagun Sharma,
  • Pankaj Kumar,
  • Pramod Verma,
  • Rajnish Sharma,
  • Naveen Chand Sharma

摘要

Walnuts hold significant importance due to their economical and nutritional value. However, walnuts possess challenges in in vitro establishment of cultures due to their recalcitrant nature. Optimizing explants, sterilization procedures, nutrient medium, and plant growth regulators, are integral parts for developing a successful micropropagation protocol. To carry out this research, different explants viz., vegetative buds (shoot tips and nodal segments, axillary buds and lateral flushes), meristems, leaf discs, and petioles were selected. Among vegetative buds, axillary buds were found to be the most effective explants for in vitro establishment and shoot initiation with a highest survival rate of 36.33 ± 0.19%, while lateral flushes were found to be more susceptible to browning and contamination and showed the highest survival rate of 09.55 ± 0.29%. Different nutrient media viz., MS (Murashige and Skoog), WPM (Woody Plant Medium), DKWc (Driver and Kuniyuki medium containing only FeEDTA), DKWm (medium containing half concentrations of both FeEDTA and FeEDDHA), and DKWf (medium containing only FeEDDHA) were tested for the establishment of vegetative buds, but DKW medium was found to be most effective in inducing shoot initiation with an average survival rate of 54.99 ± 0.33%. However, phenolic exudation and explant browning were also maximum in DKWc (49.77 ± 0.29%) and lowest in WPM medium (9.66 ± 0.11%). Explant necrosis was observed to be highest in the MS medium (74.88 ± 0.39%) followed by WPM (55.33 ± 0.50%). As DKWf medium gives the highest survival percentage with a high explant browning rate, the DKWf medium was tested with the addition of different antioxidants. It was found that a combination of PVP, activated charcoal, and phloroglucinol was the most effective, reducing explant browning from 25.00 ± 0.19% to 19.77 ± 0.29%. Apart from vegetative buds, meristems were also found to be the least susceptible with the highest survival percentage, but successful conversion to plantlets was not achieved. In vitro establishment of walnut is a complicated process, not least due to intricate physiological and biochemical properties, which include dormancy, slow growth rate, and sensitivity to tissue culture medium. Thus, this ongoing exploration highlights the complexity of walnut tissue culture establishment process and underscores the need for further research to improve the success rate of propagation.