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<p>RNAi-mediated pest control is a cutting-edge technology that has been successfully applied to suppress insect populations. <i>Bactrocera dorsalis,</i> an important fruit and vegetable pest worldwide, is becoming increasingly resistant to chemical control methods, making it urgent to explore new environmentally friendly alternatives. In this study, we used RNAi technology to screen the lethal gene <i>BdCactin</i> which had the highest lethal efficiency against adult <i>B. dorsalis</i>. Then, we confirmed the degradation of dsRNA by <i>dsRNase</i> through in vitro experiments and improved the lethal effect by silencing <i>dsRNases</i> via RNAi, thereby prolonging the duration of dsRNA. Finally, we further encapsulated dsRNA with the nanomaterial chitosan to protect dsRNA from degradation and improved mortality. Overall, this study presents a novel cost-effective chitosan-ds<i>BddsRNase</i>s-ds<i>BdCactin</i> RNAi delivery system against adult <i>B. dorsalis</i>. It lays the foundation for using RNAi technology to control <i>B. dorsalis</i> and has significant implications for exploring the application of nano-loaded RNAi targeted lethal technology in pest management.</p>

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Construction of a chitosan nanoparticle-based nucleic acid pesticide system for the control of Bactrocera dorsalis

  • 期刊论文

  • 发表日期：

    2026年1月19日

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  • Guijian Zhang,
  • Chuang Wu,
  • Ruxin Guo,
  • Bingbing Wei,
  • Shuai Cao,
  • Haoran Wang,
  • Zhen Cao,
  • Yi Liu,
  • Dandan Wei,
  • Peng Han,
  • Changying Niu

摘要

RNAi-mediated pest control is a cutting-edge technology that has been successfully applied to suppress insect populations. Bactrocera dorsalis, an important fruit and vegetable pest worldwide, is becoming increasingly resistant to chemical control methods, making it urgent to explore new environmentally friendly alternatives. In this study, we used RNAi technology to screen the lethal gene BdCactin which had the highest lethal efficiency against adult B. dorsalis. Then, we confirmed the degradation of dsRNA by dsRNase through in vitro experiments and improved the lethal effect by silencing dsRNases via RNAi, thereby prolonging the duration of dsRNA. Finally, we further encapsulated dsRNA with the nanomaterial chitosan to protect dsRNA from degradation and improved mortality. Overall, this study presents a novel cost-effective chitosan-dsBddsRNases-dsBdCactin RNAi delivery system against adult B. dorsalis. It lays the foundation for using RNAi technology to control B. dorsalis and has significant implications for exploring the application of nano-loaded RNAi targeted lethal technology in pest management.