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<p>Trehalase (TRE) is an important enzyme that is responsible for trehalose hydrolysis. However, the effect of NLTRE on the reproduction of <i>Nilaparvata lugens</i> has not been clearly reported. To comprehensively evaluate the pest control potential of NLTRE, this study analyzed the effect of NLTRE on female reproduction of <i>N. lugens</i> by inhibiting TRE with dsTREs injection at mRNA level and validamycin injection at protein level, respectively. The results showed that validamycin not only significantly reduced the female body weight, but also extended the preoviposition time, but dsTREs had no significant effect on these phenotypes. Besides, validamycin significantly inhibited the ovarian development of females in the early stage, while dsTREs affected the ovarian development in the later stage. However, both two treatments have extremely significantly reduced the total number of eggs laid by female, and the egg hatchability also was extremely significantly decreased, likely due to the destruction of chitin components in egg shells. Therefore, TRE inhibition can decrease the fecundity of <i>N. lugens</i> female, which suggest that TRE is a potential pest control target.</p>

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Trehalase regulates ovarian maturation and egg hatchability of Nilaparvata lugens

  • 期刊论文

  • 发表日期：

    2025年5月19日

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  • Yongkang Liu,
  • Yang Zhu,
  • Sijing Wan,
  • Xianzhong Wang,
  • Liwen Guan,
  • Caidi Xu,
  • Binghua Xie,
  • Shigui Wang,
  • Sisi Sun,
  • Bin Tang

摘要

Trehalase (TRE) is an important enzyme that is responsible for trehalose hydrolysis. However, the effect of NLTRE on the reproduction of Nilaparvata lugens has not been clearly reported. To comprehensively evaluate the pest control potential of NLTRE, this study analyzed the effect of NLTRE on female reproduction of N. lugens by inhibiting TRE with dsTREs injection at mRNA level and validamycin injection at protein level, respectively. The results showed that validamycin not only significantly reduced the female body weight, but also extended the preoviposition time, but dsTREs had no significant effect on these phenotypes. Besides, validamycin significantly inhibited the ovarian development of females in the early stage, while dsTREs affected the ovarian development in the later stage. However, both two treatments have extremely significantly reduced the total number of eggs laid by female, and the egg hatchability also was extremely significantly decreased, likely due to the destruction of chitin components in egg shells. Therefore, TRE inhibition can decrease the fecundity of N. lugens female, which suggest that TRE is a potential pest control target.