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<p>The purpose of the study was to synthesize bergapten-loaded nanostructured lipid carriers (NLCs) to characterize and develop an HPLC method. The bergapten-loaded NCLs were characterized for size, polydispersity index (PDI), and zeta potential (ZP). The morphology of NLCs was observed by scanning electron microscopy (SEM). The drug encapsulation and loading efficiency were evaluated. Moreover, an HPLC method was developed and validated in terms of accuracy, precision, specificity, linearity, limit of detection, and limit of quantitation. Bergapten-loaded NLCs exhibited a size of 23.5&#xa0;nm with 0.25 PDI, confirming narrow size distributions, whereas blank NLCs showed 20.7&#xa0;nm size and 0.19 PDI. The ZP of bergapten-loaded NLCs was −&#xa0;13.0&#xa0;mV, which was found to be almost the same as blank NLCs. SEM images confirmed the smooth surface and spherical shape of NLCs. The HPLC-validated method for drug quantification was found to be simple and efficient. Successful fabrication of drug-loaded NLCs showed size, PDI, and ZP within range with a smooth and spherical shape, and HPLC methods showed good estimation of bergapten in NLCs.</p>

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Characterization and Establishment of Validated Reverse-Phase HPLC Method for Bergapten-Loaded Nanostructured Lipid Carriers

  • 期刊论文

  • 发表日期：

    2025年8月4日

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  • Muneeb Ur Rahman,
  • Shumaila Arshad,
  • Mulazim Hussain Asim,
  • Alamgeer,
  • Hafiz Muhammad Irfan,
  • Nayab Tahir

摘要

The purpose of the study was to synthesize bergapten-loaded nanostructured lipid carriers (NLCs) to characterize and develop an HPLC method. The bergapten-loaded NCLs were characterized for size, polydispersity index (PDI), and zeta potential (ZP). The morphology of NLCs was observed by scanning electron microscopy (SEM). The drug encapsulation and loading efficiency were evaluated. Moreover, an HPLC method was developed and validated in terms of accuracy, precision, specificity, linearity, limit of detection, and limit of quantitation. Bergapten-loaded NLCs exhibited a size of 23.5 nm with 0.25 PDI, confirming narrow size distributions, whereas blank NLCs showed 20.7 nm size and 0.19 PDI. The ZP of bergapten-loaded NLCs was − 13.0 mV, which was found to be almost the same as blank NLCs. SEM images confirmed the smooth surface and spherical shape of NLCs. The HPLC-validated method for drug quantification was found to be simple and efficient. Successful fabrication of drug-loaded NLCs showed size, PDI, and ZP within range with a smooth and spherical shape, and HPLC methods showed good estimation of bergapten in NLCs.