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<p><i>Choerospondias axillaris (C. axillaris)</i>, recognized as an effective herbal remedy for coronary heart disease (CHD), has been clinically utilized. Although the angiotensin-converting enzyme (ACE) has been extensively investigated as a pertinent target for CHD treatment, there is currently limited research on screening potential ACE inhibitors from <i>C. axillaris</i>. To advance the broader utilization of <i>C. axillaris</i> in coronary heart disease treatment, the target active components of ACE in <i>C. axillaris</i> were analyzed through the integration of magnetic-targeted fishing technology with functionalized magnetic nanospheres. Magnetic nanospheres functionalized with GO@Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>-ACE (SMGO-ACE) were characterized using a vibrating sample magnetometer (VSM), scanning electron microscopy (SEM), and transmission electron microscope (TEM). The specific binding of lisinopril with the angiotensin-converting enzyme was employed to optimize the performance of the synthesized material and the relevant conditions during targeted fishing. Subsequently, five active ingredients were identified as (2R,3S)-Dihydrodehydroconiferyl alcohol, isovanillin, quinic acid, chrysin, and isorhamnetin. This study provides a precedent for the targeted extraction and separation of active ingredients in complex mixtures.</p>

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Affinity Fishing of ACE Ligands from C. axillaris using Surface Functionalized Magnetic Nanoparticles

  • 期刊论文

  • 发表日期：

    2025年6月17日

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  • Xianhong Zhu,
  • Min Zhang,
  • Kunming Qin,
  • Xueying Qin,
  • Miaomiao Chi,
  • Yan Zhang,
  • Hongsen Wang,
  • Xichen Song,
  • Xun Gao

摘要

Choerospondias axillaris (C. axillaris), recognized as an effective herbal remedy for coronary heart disease (CHD), has been clinically utilized. Although the angiotensin-converting enzyme (ACE) has been extensively investigated as a pertinent target for CHD treatment, there is currently limited research on screening potential ACE inhibitors from C. axillaris. To advance the broader utilization of C. axillaris in coronary heart disease treatment, the target active components of ACE in C. axillaris were analyzed through the integration of magnetic-targeted fishing technology with functionalized magnetic nanospheres. Magnetic nanospheres functionalized with GO@Fe3O4@SiO2-ACE (SMGO-ACE) were characterized using a vibrating sample magnetometer (VSM), scanning electron microscopy (SEM), and transmission electron microscope (TEM). The specific binding of lisinopril with the angiotensin-converting enzyme was employed to optimize the performance of the synthesized material and the relevant conditions during targeted fishing. Subsequently, five active ingredients were identified as (2R,3S)-Dihydrodehydroconiferyl alcohol, isovanillin, quinic acid, chrysin, and isorhamnetin. This study provides a precedent for the targeted extraction and separation of active ingredients in complex mixtures.