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Objective <p>This study investigates the dynamic metabolic characteristics of glioblastoma (GBM) using Deuterium Metabolic Imaging (DMI) at 7&#xa0;T, aiming to dynamically characterize the Warburg effect in vivo.</p> Material and methods <p>Five newly diagnosed GBM patients underwent dynamic DMI prior to any treatment. 3D <sup>2</sup>H free-induction-decay (FID)-Magnetic resonance spectroscopy imaging (MRSI) measurements (11:44&#xa0;min per scan) were performed at 7&#xa0;T during ~ 100&#xa0;min following [6,6’-<sup>2</sup>H<sub>2</sub>]glucose consumption. Venous plasma glucose and plasma <sup>2</sup>H-Glc atom percent enrichment (APE) levels were measured during the scan. Brain <sup>2</sup>H-glucose (<sup>2</sup>H-Glc),<sup>2</sup>H-Glutamate/Glutamine (<sup>2</sup>H-Glx), <sup>2</sup>H-Lactate (<sup>2</sup>H-Lac), <sup>2</sup>H-Lac/<sup>2</sup>H-Glx were analyzed with a two-level (time and tissue type) Linear Mixed Model.</p> Results <p>Brain <sup>2</sup>H-Glc levels were similar across tissue types. <sup>2</sup>H-Glx was significantly lower in tumors compared to normal appearing brain tissue (NABT) (<i>p</i> &lt; 0.01). <sup>2</sup>H-Lac was significantly higher in tumors compared to NABT (<i>P</i> &lt; 0.01). The <sup>2</sup>H-Lac/<sup>2</sup>H-Glx ratio provided tumor-specific contrast, starting 40-50&#xa0;min post [6,6’-<sup>2</sup>H<sub>2</sub>]glucose consumption. Venous plasma glucose and <sup>2</sup>H-Glc APE increased within 50&#xa0;min and venous <sup>2</sup>H-Glc APE stabilized at ~ 60%.</p> Discussion <p>Dynamic DMI at 7&#xa0;T reveals metabolic alterations in GBM, particularly through the <sup>2</sup>H-Lac/<sup>2</sup>H-Glx ratio. This contrast was primarily driven by decreased <sup>2</sup>H-Glx rather than profoundly increased <sup>2</sup>H-Lac. These findings support the utility of DMI in assessing metabolic reprogramming in brain tumors.</p>

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Dynamic deuterium metabolic imaging in glioblastoma at 7T

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月9日

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  • Narjes Ahmadian,
  • Mark Gosselink,
  • Sigrid Otto,
  • Dimitri Welting,
  • Kiki Tesselaar,
  • Tom Snijders,
  • Pieter van Eijsden,
  • Jeanine Prompers,
  • Dennis Klomp,
  • Evita Wiegers

摘要

Objective

This study investigates the dynamic metabolic characteristics of glioblastoma (GBM) using Deuterium Metabolic Imaging (DMI) at 7 T, aiming to dynamically characterize the Warburg effect in vivo.

Material and methods

Five newly diagnosed GBM patients underwent dynamic DMI prior to any treatment. 3D 2H free-induction-decay (FID)-Magnetic resonance spectroscopy imaging (MRSI) measurements (11:44 min per scan) were performed at 7 T during ~ 100 min following [6,6’-2H2]glucose consumption. Venous plasma glucose and plasma 2H-Glc atom percent enrichment (APE) levels were measured during the scan. Brain 2H-glucose (2H-Glc),2H-Glutamate/Glutamine (2H-Glx), 2H-Lactate (2H-Lac), 2H-Lac/2H-Glx were analyzed with a two-level (time and tissue type) Linear Mixed Model.

Results

Brain 2H-Glc levels were similar across tissue types. 2H-Glx was significantly lower in tumors compared to normal appearing brain tissue (NABT) (p < 0.01). 2H-Lac was significantly higher in tumors compared to NABT (P < 0.01). The 2H-Lac/2H-Glx ratio provided tumor-specific contrast, starting 40-50 min post [6,6’-2H2]glucose consumption. Venous plasma glucose and 2H-Glc APE increased within 50 min and venous 2H-Glc APE stabilized at ~ 60%.

Discussion

Dynamic DMI at 7 T reveals metabolic alterations in GBM, particularly through the 2H-Lac/2H-Glx ratio. This contrast was primarily driven by decreased 2H-Glx rather than profoundly increased 2H-Lac. These findings support the utility of DMI in assessing metabolic reprogramming in brain tumors.