Scholarlink

<p>A loop-mediated isothermal amplification (LAMP) assay that was developed for <i>Agroathelia rolfsii</i> (syn. <i>Sclerotium rolfsii</i>) gave a positive reaction for <i>A. rolfsii</i>, but not <i>Calonectria ilicicola</i> or <i>Phytophthora</i> species. The LAMP reaction with 100 fg or more of <i>A. rolfsii</i> DNA was completed in 1 h. The LAMP assay was 1000 times more sensitive than conventional PCR and detected <i>A. rolfsii</i>-infected soybean seedlings in fields. The technique provides a rapid, simplified diagnosis for disease management.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Rapid, simplified LAMP assay to detect Agroathelia rolfsii (syn. Sclerotium rolfsii) in black soybean with damping off

  • 期刊论文

  • 发表日期：

    2025年10月13日

查看全文
  • Ken Harata,
  • Yusuke Momma,
  • Susumu Tokumaru

摘要

A loop-mediated isothermal amplification (LAMP) assay that was developed for Agroathelia rolfsii (syn. Sclerotium rolfsii) gave a positive reaction for A. rolfsii, but not Calonectria ilicicola or Phytophthora species. The LAMP reaction with 100 fg or more of A. rolfsii DNA was completed in 1 h. The LAMP assay was 1000 times more sensitive than conventional PCR and detected A. rolfsii-infected soybean seedlings in fields. The technique provides a rapid, simplified diagnosis for disease management.