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<p>Sweet potato foot rot is a serious fungal disease threatening production. For faster detection of the fungus, we adapted a real-time PCR method to test bulk samples simultaneously. We estimated the probability of detection (POD) and calculated optimal subgroup numbers and sample sizes. Lowest detection rate for the PCR was approximately 81% for tubers and 83% for stems. Based on a POD of 81%, required sample sizes were determined for the impermissible contamination thresholds of infected plants of 1% and 0.1%. This method supports efficient monitoring and sustainable production of sweet potatoes.</p>

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Bulk sample testing with real-time PCR to detect Diaporthe destruens in sweet potato seedlings

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • Takashi Fujikawa,
  • Hiroe Hatomi,
  • Kohji Yamamura,
  • Yoshifumi Tsukiori,
  • Yasuhiro Inoue,
  • Kazuki Fujiwara

摘要

Sweet potato foot rot is a serious fungal disease threatening production. For faster detection of the fungus, we adapted a real-time PCR method to test bulk samples simultaneously. We estimated the probability of detection (POD) and calculated optimal subgroup numbers and sample sizes. Lowest detection rate for the PCR was approximately 81% for tubers and 83% for stems. Based on a POD of 81%, required sample sizes were determined for the impermissible contamination thresholds of infected plants of 1% and 0.1%. This method supports efficient monitoring and sustainable production of sweet potatoes.