<p>Ninety-nine <i>Corynespora cassiicola</i> isolates were collected from eggplant fields in Kochi Prefecture, Japan, in 2020–2023, and tested for their sensitivity to pyribencarb and azoxystrobin. Sequence analysis of the <i>cytochrome</i> <i>b</i> gene revealed that 68 isolates harbored the G143A substitution mutation. Inoculation tests using potted eggplants showed that the G143A-harboring isolates were cross-resistant to pyribencarb and azoxystrobin, although disease severity on plants treated with pyribencarb was lower than on those treated with azoxystrobin. In vitro sensitivity tests using mycelial homogenates showed that sensitivity of G143A-harboring isolates against pyribencarb and azoxystrobin was lower than that of wild-type isolates. The proportion of resistant isolates collected in 2022 and 2023 was higher than among isolates collected in 2020 and 2021.</p>

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Cross-resistance between pyribencarb and azoxystrobin in eggplant isolates of Corynespora Cassiicolla that have the G143A mutation in Kochi prefecture, Japan

  • Yoshifumi Shimomoto,
  • Yuka Morizane,
  • Ryotaro Okada,
  • Tomoyuki Okada

摘要

Ninety-nine Corynespora cassiicola isolates were collected from eggplant fields in Kochi Prefecture, Japan, in 2020–2023, and tested for their sensitivity to pyribencarb and azoxystrobin. Sequence analysis of the cytochrome b gene revealed that 68 isolates harbored the G143A substitution mutation. Inoculation tests using potted eggplants showed that the G143A-harboring isolates were cross-resistant to pyribencarb and azoxystrobin, although disease severity on plants treated with pyribencarb was lower than on those treated with azoxystrobin. In vitro sensitivity tests using mycelial homogenates showed that sensitivity of G143A-harboring isolates against pyribencarb and azoxystrobin was lower than that of wild-type isolates. The proportion of resistant isolates collected in 2022 and 2023 was higher than among isolates collected in 2020 and 2021.