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Objective <p>To assess the influence of tooth stained with different colored solutions on the cytotoxicity of a 35% H<sub>2</sub>O<sub>2</sub>-bleaching gel.</p> Materials and methods <p>The established groups were (<i>n</i> = 8): NC– without treatment (negative control); PC– 35% H<sub>2</sub>O<sub>2</sub> (positive control); CO– coffee + 35% H<sub>2</sub>O<sub>2</sub>; BT– black tea + 35% H<sub>2</sub>O<sub>2</sub>; WI– wine + 35% H<sub>2</sub>O<sub>2</sub>; COBTWI– coffee/black tea/wine + 35% H<sub>2</sub>O<sub>2</sub>. Standardized enamel/dentin disks were submitted to staining protocols, adapted to artificial pulp chambers, and bleaching was performed. The chromatic alterations (ΔE<sub>00,</sub> ΔWI<sub>D</sub>) were assessed after bleaching and the extracts (culture medium + diffused gel components) were collected and applied to odontoblast-like cells. The amount of diffused H<sub>2</sub>O<sub>2</sub>, cell viability, and oxidative stress were evaluated and data were submitted to ANOVA/Tukey; <i>p</i> &lt; 0.05).</p> Results <p>Thirty five% H<sub>2</sub>O<sub>2</sub> effectively bleached the stained specimens, regardless of the staining solution used (CO, BT<sub>,</sub> WI, and COBTWI), and no differences were found among the stained protocols (<i>p</i> &gt; 0.05). All staining protocols reduced H<sub>2</sub>O<sub>2</sub> trans-amelodentinal diffusion (<i>p</i> &gt; 0.05), decreasing bleaching cytotoxicity in comparison to PC (<i>p</i> &lt; 0.05).</p> Conclusion <p>Irrespective of the nature of the pigment used for staining enamel and dentin, the gel with 35% H<sub>2</sub>O<sub>2</sub> effectively bleached the tooth structure. The presence of pigments in both dental tissues, regardless their nature, reduced the trans-amelodentinal diffusion of H₂O₂ and consequently the cytotoxicity of the bleaching gel.</p>

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Can pigments of different natures interfere with the cytotoxicity from in-office bleaching?

  • 期刊论文

  • 发表日期：

    2025年2月13日

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  • Rafael Antonio de Oliveira Ribeiro,
  • Beatriz Voss Martins,
  • Marlon Ferreira Dias,
  • Victória Peruchi,
  • Caroline Anselmi,
  • Igor Paulino Mendes Soares,
  • Josimeri Hebling,
  • Vanessa Cavalli,
  • Carlos Alberto de Souza Costa

摘要

Objective

To assess the influence of tooth stained with different colored solutions on the cytotoxicity of a 35% H2O2-bleaching gel.

Materials and methods

The established groups were (n = 8): NC– without treatment (negative control); PC– 35% H2O2 (positive control); CO– coffee + 35% H2O2; BT– black tea + 35% H2O2; WI– wine + 35% H2O2; COBTWI– coffee/black tea/wine + 35% H2O2. Standardized enamel/dentin disks were submitted to staining protocols, adapted to artificial pulp chambers, and bleaching was performed. The chromatic alterations (ΔE00, ΔWID) were assessed after bleaching and the extracts (culture medium + diffused gel components) were collected and applied to odontoblast-like cells. The amount of diffused H2O2, cell viability, and oxidative stress were evaluated and data were submitted to ANOVA/Tukey; p < 0.05).

Results

Thirty five% H2O2 effectively bleached the stained specimens, regardless of the staining solution used (CO, BT, WI, and COBTWI), and no differences were found among the stained protocols (p > 0.05). All staining protocols reduced H2O2 trans-amelodentinal diffusion (p > 0.05), decreasing bleaching cytotoxicity in comparison to PC (p < 0.05).

Conclusion

Irrespective of the nature of the pigment used for staining enamel and dentin, the gel with 35% H2O2 effectively bleached the tooth structure. The presence of pigments in both dental tissues, regardless their nature, reduced the trans-amelodentinal diffusion of H₂O₂ and consequently the cytotoxicity of the bleaching gel.