<p><i>Vanderhorstia supersaiyan</i> sp. nov. (Perciformes: Gobiidae) is described from a single specimen (77.0 mm in standard length) collected from 210 m depth off Ishigaki-jima Island, Okinawa, Japan. The new species has two distinct yellow longitudinal stripes on the second dorsal, anal, and caudal fins, which are unique features within the genus. The new species can also be clearly distinguished from congeners by other coloration features, including faint yellow spots on the head and dorsal body margin, distinct yellow blotches and yellow upper margin on the first dorsal fin, and four faint yellowish-brown diamond-shaped blotches laterally on the body. A combination of meristic and morphometric characters also set the new species apart from congeners, all of which inhabit significantly shallower depths. The study documents a new discovery in the twilight zone highlighting the value of advancing research in this little studied habitat.</p>