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<p><i>Lethenteron satoi</i> is a species of petromyzontid lampreys recently described from the Ishikari River system in Hokkaido, Japan. Although this species was clearly distinguishable based on mitochondrial DNA (mtDNA) sequences and a combination of morphological characteristics, its species status required further confirmation using nuclear DNA evidence. We examined the phylogenetic independence and placement of <i>L. satoi</i> among the five Japanese <i>Lethenteron</i> species using SNP markers obtained via the multiplexed ISSR genotyping-by-sequencing (MIG-seq) method. Admixture and network analyses clearly indicated that <i>L. satoi</i> is a genetically distinct species and clusters more closely with <i>Lethenteron camtschaticum</i>, <i>Lethenteron reissneri</i>, and <i>Lethenteron mitsukurii</i> than with <i>Lethenteron hattai</i>, consistent with the mtDNA phylogeny. We also provided mitochondrial 12S rRNA sequences of the five Japanese <i>Lethenteron</i> species for environmental DNA metabarcoding, confirming their utility in distinguishing <i>L. satoi</i> from other species.</p>

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The genetic distinctiveness of Lethenteron satoi (Petromyzontidae) confirmed by nuclear SNP markers

  • 期刊论文

  • 发表日期：

    2025年9月23日

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  • Katsutoshi Watanabe,
  • Kyoko Ikeuchi,
  • Yugo Miuchi,
  • Harumi Sakai,
  • Akihisa Iwata

摘要

Lethenteron satoi is a species of petromyzontid lampreys recently described from the Ishikari River system in Hokkaido, Japan. Although this species was clearly distinguishable based on mitochondrial DNA (mtDNA) sequences and a combination of morphological characteristics, its species status required further confirmation using nuclear DNA evidence. We examined the phylogenetic independence and placement of L. satoi among the five Japanese Lethenteron species using SNP markers obtained via the multiplexed ISSR genotyping-by-sequencing (MIG-seq) method. Admixture and network analyses clearly indicated that L. satoi is a genetically distinct species and clusters more closely with Lethenteron camtschaticum, Lethenteron reissneri, and Lethenteron mitsukurii than with Lethenteron hattai, consistent with the mtDNA phylogeny. We also provided mitochondrial 12S rRNA sequences of the five Japanese Lethenteron species for environmental DNA metabarcoding, confirming their utility in distinguishing L. satoi from other species.