<p>University websites offer a wide range of information presented in multiple formats, necessitating compatibility and accessibility for every user. This paper evaluates the accessibility of the top 25 world and the top 25 QS-ranked Indian universities using WCAG 2.2. This research uses SortSite, an automatic web accessibility and user-friendly web testing tool to efficiently checks websites for accessibility, usability, browser compatibility, and search engine optimization (SEO) issues was used to assess for accessibility. Among the Indian universities, ‘.ac.in’ is the most used TLD, while among world universities, ‘.edu’ is the most used TLD. Indian universities have the greatest number of violations at each conformance level (A, AA, and AAA) compared with world universities. The content on these websites is not very presentable, understandable, interactive and user-friendly, as the largest number of violations among all principles were found at conformance level A, followed by AAA and AA. Indian universities have the most common failures at conformance levels A and AAA while world universities have the most common failures at conformance level AA, caused by erroneous implementations of success criteria, more specifically related to CSS and HTML. Indian institutions have more compatibility issues than world universities. Compared to iOS, Safari, Android, Chrome, and Opera, the web browser Firefox was determined to be the most incompatible, while Edge was shown to be the most compatible.</p>