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<p>Climate change driven by increasing greenhouse gas emissions has intensified the demand for technologies that can reduce and utilize these gases. Biological methane reduction and utilization technologies are gaining attention due to their environmental sustainability. In this study, we describe the methane-based biosynthesis of D-pantothenic acid (vitamin B5) using a newly isolated Type II methanotroph, <i>Methylosinus sporium</i> UOS2. Genome analysis of the UOS2 strain, isolated from wastewater, revealed a complete D-pantothenic acid biosynthetic pathway. Cultivation with methane as the sole carbon source confirmed its ability to produce D-pantothenic acid. Optimization of culture conditions resulted in a 55.3% increase in biomass and a 37.8% increase in D-pantothenic acid production (0.37&#xa0;mg/L) compared to cultivation in ammonium-based medium. Furthermore, overexpression of <i>panE</i>, encoding ketopantoate reductase, led to a 3.5-fold increase in D-pantothenic acid production (1.29&#xa0;mg/L) within 96&#xa0;h. These findings demonstrate the feasibility of direct methane-to-vitamin conversion using <i>M. sporium</i> UOS2 and highlight that medium optimization combined with targeted gene overexpression is effective strategies to enhance production, laying the groundwork for future methane-based biorefineries.</p>

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Sustainable D-pantothenic acid production from methane using a newly isolated methanotrophic bacterium, Methylosinus sporium UOS2

  • 期刊论文

  • 发表日期：

    2026年5月25日

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  • Min Sun Kim,
  • Yong Jun Choi

摘要

Climate change driven by increasing greenhouse gas emissions has intensified the demand for technologies that can reduce and utilize these gases. Biological methane reduction and utilization technologies are gaining attention due to their environmental sustainability. In this study, we describe the methane-based biosynthesis of D-pantothenic acid (vitamin B5) using a newly isolated Type II methanotroph, Methylosinus sporium UOS2. Genome analysis of the UOS2 strain, isolated from wastewater, revealed a complete D-pantothenic acid biosynthetic pathway. Cultivation with methane as the sole carbon source confirmed its ability to produce D-pantothenic acid. Optimization of culture conditions resulted in a 55.3% increase in biomass and a 37.8% increase in D-pantothenic acid production (0.37 mg/L) compared to cultivation in ammonium-based medium. Furthermore, overexpression of panE, encoding ketopantoate reductase, led to a 3.5-fold increase in D-pantothenic acid production (1.29 mg/L) within 96 h. These findings demonstrate the feasibility of direct methane-to-vitamin conversion using M. sporium UOS2 and highlight that medium optimization combined with targeted gene overexpression is effective strategies to enhance production, laying the groundwork for future methane-based biorefineries.