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<p><i>Eucommia ulmoides</i> is a cold-tolerant rubber-producing tree capable of synthesizing trans-polyisoprene rubber in its leaves, bark, and fruit pericarp. The WRKY family constitutes a major class of plant transcriptional regulators and is widely implicated in controlling specialized metabolic pathways. However, for most <i>E. ulmoides WRKY</i> genes, their regulatory contributions to rubber formation are still poorly defined. In this study, we obtained the full-length <i>EuWRKY1</i> cDNA from <i>E. ulmoides</i> by RACE and assessed its function using genetic transformation. Sequence alignment revealed that <i>EuWRKY1</i> shares 62.37% homology with <i>HbWRKY1</i>, a key regulator of rubber biosynthesis in <i>Hevea brasiliensis</i>. Subcellular localization analysis showed that <i>EuWRKY1</i> is predominantly nuclear, and transcriptional activation assays confirmed its transactivation capability. Overexpression of <i>EuWRKY1</i> in <i>E. ulmoides</i> significantly increased rubber filament and rubber particle numbers and elevated leaf rubber content, accompanied by markedly higher expression of key rubber biosynthesis-related genes, including <i>EuSRPP6</i> and <i>EuSRPP7</i>. These results indicate that <i>EuWRKY1</i> acts as a positive regulator of rubber biosynthesis in <i>E. ulmoides</i>, providing new insights into the transcriptional regulatory network underlying trans-polyisoprene formation and offering a potential target for molecular improvement to enhance rubber yield.</p> Graphical Abstract <p></p>

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Positive regulatory role of EuWRKY1 in the biosynthesis of Eucommia ulmoides rubber

  • 期刊论文

  • 发表日期：

    2026年7月1日

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  • Juanying Wang,
  • Ju Cai,
  • Yin You,
  • Xiuliu Yang,
  • Dandan Yuan,
  • Yan Li

摘要

Eucommia ulmoides is a cold-tolerant rubber-producing tree capable of synthesizing trans-polyisoprene rubber in its leaves, bark, and fruit pericarp. The WRKY family constitutes a major class of plant transcriptional regulators and is widely implicated in controlling specialized metabolic pathways. However, for most E. ulmoides WRKY genes, their regulatory contributions to rubber formation are still poorly defined. In this study, we obtained the full-length EuWRKY1 cDNA from E. ulmoides by RACE and assessed its function using genetic transformation. Sequence alignment revealed that EuWRKY1 shares 62.37% homology with HbWRKY1, a key regulator of rubber biosynthesis in Hevea brasiliensis. Subcellular localization analysis showed that EuWRKY1 is predominantly nuclear, and transcriptional activation assays confirmed its transactivation capability. Overexpression of EuWRKY1 in E. ulmoides significantly increased rubber filament and rubber particle numbers and elevated leaf rubber content, accompanied by markedly higher expression of key rubber biosynthesis-related genes, including EuSRPP6 and EuSRPP7. These results indicate that EuWRKY1 acts as a positive regulator of rubber biosynthesis in E. ulmoides, providing new insights into the transcriptional regulatory network underlying trans-polyisoprene formation and offering a potential target for molecular improvement to enhance rubber yield.

Graphical Abstract