Scholarlink

<p>Oat anthracnose, primarily caused by <i>Colletotrichum cereale</i>, represents a significant threat to oat production, necessitating the development of sustainable biocontrol alternatives. In this study, we characterized an oat endophytic bacterium, <i>Bacillus velezensis</i> JN.Y2, isolated from healthy oat leaves in Inner Mongolia. In vitro assays demonstrated that <i>B. velezensis</i> JN.Y2 (designated as Bv. JN.Y2) possesses an inhibitory activity against <i>C. cereale</i> (74.49%) and exhibits a broad antifungal spectrum. Greenhouse and three-year multi-location field trials confirmed its beneficial biocontrol efficacy, which remained stable even under fluctuating climatic conditions and high disease pressure, consistently outperforming conventional chemical treatments. Beyond disease suppression, Bv. JN.Y2 significantly enhanced oat growth and grain yield, supported by its ability to produce IAA, solubilize nutrients, and secrete diverse hydrolytic enzymes. Complete genome sequencing revealed a 3.87&#xa0;Mb circular chromosome containing 13 secondary metabolite BGCs and 4 AOIs for RiPPs, including Amylocyclicin and LCI. Comparative genomic analysis highlighted an “open” pangenome and identified 411 unique genes associated with specialized metabolism and environmental sensing. While Bv. JN.Y2 shares high sequence synteny with <i>B. velezensis</i> CBMB205, distinct variations in the sporulation kinase <i>kinA</i> and secondary metabolite pathways suggest a fine-tuned adaptation to the oat endosphere. Furthermore, biosafety evaluations confirmed a relatively high level of genetic stability and a lack of active antibiotic resistance. Collectively, these findings provide a beneficial molecular foundation for the application of Bv. JN.Y2 as a reliable and secure biocontrol agent in sustainable agriculture.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Whole-genome and Comparative Genomic Analysis Reveal the Biocontrol and Plant Growth-Promoting Potential of Bacillus velezensis JN.Y2 Against Oat Anthracnose

  • 期刊论文

  • 发表日期：

    2026年6月26日

查看全文
  • Wei Quan,
  • Ting Zhang,
  • Chen-Lu Liu,
  • Shao-xuan Shi,
  • Xi-xi Zhang,
  • Ke-han Liu,
  • Chun-Yang Wang,
  • Ming-min Zhao,
  • Bao-Zhu Dong,
  • Hong-You Zhou

摘要

Oat anthracnose, primarily caused by Colletotrichum cereale, represents a significant threat to oat production, necessitating the development of sustainable biocontrol alternatives. In this study, we characterized an oat endophytic bacterium, Bacillus velezensis JN.Y2, isolated from healthy oat leaves in Inner Mongolia. In vitro assays demonstrated that B. velezensis JN.Y2 (designated as Bv. JN.Y2) possesses an inhibitory activity against C. cereale (74.49%) and exhibits a broad antifungal spectrum. Greenhouse and three-year multi-location field trials confirmed its beneficial biocontrol efficacy, which remained stable even under fluctuating climatic conditions and high disease pressure, consistently outperforming conventional chemical treatments. Beyond disease suppression, Bv. JN.Y2 significantly enhanced oat growth and grain yield, supported by its ability to produce IAA, solubilize nutrients, and secrete diverse hydrolytic enzymes. Complete genome sequencing revealed a 3.87 Mb circular chromosome containing 13 secondary metabolite BGCs and 4 AOIs for RiPPs, including Amylocyclicin and LCI. Comparative genomic analysis highlighted an “open” pangenome and identified 411 unique genes associated with specialized metabolism and environmental sensing. While Bv. JN.Y2 shares high sequence synteny with B. velezensis CBMB205, distinct variations in the sporulation kinase kinA and secondary metabolite pathways suggest a fine-tuned adaptation to the oat endosphere. Furthermore, biosafety evaluations confirmed a relatively high level of genetic stability and a lack of active antibiotic resistance. Collectively, these findings provide a beneficial molecular foundation for the application of Bv. JN.Y2 as a reliable and secure biocontrol agent in sustainable agriculture.