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<p>Plant transcription factors are essential for stress adaptation, regulating responses to both abiotic and biotic stimuli. Among these factors, Teosinte Branched 1, Cycloidea, and PCF (TCP) play key roles in modulating plant responses to abiotic stress. In this study, the specific roles of TCP1 from <i>Liriodendron chinense</i> were examined through gene expression analysis. LchiTCP1 was isolated and transformed into Arabidopsis; its expression under drought, cold, and salt stress conditions was monitored. Transgenic Arabidopsis plants carrying the LchiTCP1 gene showed enhanced growth and root development with distinctive features. Biochemical analyses indicated that LchiTCP1 confers abiotic stress tolerance. Notably, plants overexpressing LchiTCP1 exhibited higher chlorophyll levels after exposure to various stress conditions than wild-type (WT) plants. Histochemical staining of leaves from transgenic plants with DAB and NBT showed reduced staining relative to WT, suggesting lower reactive oxygen species (ROS) activity in TCP1-overexpressing plants. To confirm these findings, the levels of SOD, POD, and CAT enzymes were significantly lower in OE-LchiTCP1 stressed plants than in WT plants. Conversely, OE-LchiTCP1 plants showed at least a twofold increase in MDA content relative to WT plants. qPCR analysis across different stresses revealed increased expression of stress-responsive genes in LchiTCP1-overexpressing plants compared to WT. Therefore, we conclude that the TCP1 gene in <i>L. chinense</i> regulates responses to cold, drought, and salt stress through interactions with downstream stress-responsive genes. This study enhances our understanding of how plants respond to abiotic stresses.</p>

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Transcription factor LchiTCP1 modulates abiotic stress response in arabidopsis

  • 期刊论文

  • 发表日期：

    2026年4月18日

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  • Hwarari Delight,
  • Yinyue Zuo,
  • Yao Zhang,
  • Xinyi Cao,
  • Tian Min,
  • Jinhui Chen,
  • Liming Yang

摘要

Plant transcription factors are essential for stress adaptation, regulating responses to both abiotic and biotic stimuli. Among these factors, Teosinte Branched 1, Cycloidea, and PCF (TCP) play key roles in modulating plant responses to abiotic stress. In this study, the specific roles of TCP1 from Liriodendron chinense were examined through gene expression analysis. LchiTCP1 was isolated and transformed into Arabidopsis; its expression under drought, cold, and salt stress conditions was monitored. Transgenic Arabidopsis plants carrying the LchiTCP1 gene showed enhanced growth and root development with distinctive features. Biochemical analyses indicated that LchiTCP1 confers abiotic stress tolerance. Notably, plants overexpressing LchiTCP1 exhibited higher chlorophyll levels after exposure to various stress conditions than wild-type (WT) plants. Histochemical staining of leaves from transgenic plants with DAB and NBT showed reduced staining relative to WT, suggesting lower reactive oxygen species (ROS) activity in TCP1-overexpressing plants. To confirm these findings, the levels of SOD, POD, and CAT enzymes were significantly lower in OE-LchiTCP1 stressed plants than in WT plants. Conversely, OE-LchiTCP1 plants showed at least a twofold increase in MDA content relative to WT plants. qPCR analysis across different stresses revealed increased expression of stress-responsive genes in LchiTCP1-overexpressing plants compared to WT. Therefore, we conclude that the TCP1 gene in L. chinense regulates responses to cold, drought, and salt stress through interactions with downstream stress-responsive genes. This study enhances our understanding of how plants respond to abiotic stresses.