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<p>The larvae of <i>Hyphantria cunea</i> are highly polyphagous, infesting a wide range of host plants, including fruit trees, ornamental species, and agroforestry crops. In this study, we evaluated the insect resistance of 10 transgenic poplar lines (B1-B10) co-expressing the <i>Cry1Ac</i> and <i>SCK</i> genes. Results showed that the mortality rate of <i>H. cunea</i> larvae fed on leaves from these transgenic lines was significantly higher than that of larvae fed on wild-type leaves. Notably, the expression levels of the <i>Cry1Ac</i> and <i>SCK</i> genes varied among the different transgenic lines. To further investigate whether the <i>Cry1Ac</i> + <i>SCK</i> transgenic lines alter internal gene expression and metabolic profiles in poplar, we conducted transcriptomic and metabolomic analyses. Transcriptome analysis revealed that the B5 transgenic line, which demonstrated high insect resistance, exhibited the greatest number of differentially expressed genes (DEGs). These DEGs were primarily enriched in pathways related to lipid and membrane metabolism, flavonoid biosynthesis, and plant hormone signal transduction. Metabolome analysis indicated that the differentially accumulated metabolites (DAMs) in the B5 line were largely associated with plant hormone signal transduction and secondary metabolic pathways. Significantly, integrated multi-omics analyses highlighted substantial enrichment in both plant hormone signal transduction and flavonoid biosynthesis pathways. This study systematically identifies key metabolic pathways and defense-related genes in <i>Cry1Ac</i> + <i>SCK</i> transgenic poplars, providing a theoretical foundation for developing next-generation insect-resistant trees through targeted genetic engineering.</p>

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Transcriptomic and metabolomic analysis of Cry1AC + SCK transgenic poplars

  • 期刊论文

  • 发表日期：

    2026年1月20日

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  • Jiahui Jiang,
  • Zhanling Sun,
  • Gaofeng Fan,
  • Boru Zhou,
  • Tingbo Jiang

摘要

The larvae of Hyphantria cunea are highly polyphagous, infesting a wide range of host plants, including fruit trees, ornamental species, and agroforestry crops. In this study, we evaluated the insect resistance of 10 transgenic poplar lines (B1-B10) co-expressing the Cry1Ac and SCK genes. Results showed that the mortality rate of H. cunea larvae fed on leaves from these transgenic lines was significantly higher than that of larvae fed on wild-type leaves. Notably, the expression levels of the Cry1Ac and SCK genes varied among the different transgenic lines. To further investigate whether the Cry1Ac + SCK transgenic lines alter internal gene expression and metabolic profiles in poplar, we conducted transcriptomic and metabolomic analyses. Transcriptome analysis revealed that the B5 transgenic line, which demonstrated high insect resistance, exhibited the greatest number of differentially expressed genes (DEGs). These DEGs were primarily enriched in pathways related to lipid and membrane metabolism, flavonoid biosynthesis, and plant hormone signal transduction. Metabolome analysis indicated that the differentially accumulated metabolites (DAMs) in the B5 line were largely associated with plant hormone signal transduction and secondary metabolic pathways. Significantly, integrated multi-omics analyses highlighted substantial enrichment in both plant hormone signal transduction and flavonoid biosynthesis pathways. This study systematically identifies key metabolic pathways and defense-related genes in Cry1Ac + SCK transgenic poplars, providing a theoretical foundation for developing next-generation insect-resistant trees through targeted genetic engineering.