Scholarlink

<p>In this study, the mitogenomes of nine species in the subfamily Cypripedioideae were newly sequenced and assembled using both short and long reads for evolutionary analyses. Complete multi-chromosomal mitogenomes were obtained for <i>Cypripedium subtropicum</i>, <i>C. henryi, Phragmipedium humboldtii</i>, <i>Phr. kovachii,</i> and <i>Paphiopedilum micranthum</i>, and draft assemblies were obtained for four additional <i>Paphiopedilum</i> species. Thirty-nine protein-coding genes were annotated and shared in nine sampled species. <i>sdh4</i> was discovered in all species of Cypripedioideae, and <i>rpl10</i> was detected in four species of <i>Paphiopedilum</i>. These two genes might have been horizontally transferred from non-orchid plants at different times. Approximately 101 to 998 repeat sequences were identified with total lengths of 417,136 to 785,960 bp in the mitogenomes of Cypripedioideae. There were 634 and 662 RNA editing sites in <i>C. subtropicum</i> and <i>Pa. gratrixianum</i>, respectively, and C-to-U editing was dominant. The <i>nad</i> and <i>ccm</i> genes exhibited high frequencies of RNA editing. Our study revealed the complexity of orchid mitogenomes, including evidence for the horizontal transfer of <i>rpl10</i> and <i>sdh4.</i></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Mitochondrial genome evolution in the orchid subfamily Cypripedioideae (Orchidaceae)

  • 期刊论文

  • 发表日期：

    2025年4月30日

查看全文
  • Shiyu Qin,
  • Hanchen Wang,
  • Miaomiao Wang,
  • Bingyi Shao,
  • Chongbo Ma,
  • Boyun Yang,
  • Xiaohua Jin

摘要

In this study, the mitogenomes of nine species in the subfamily Cypripedioideae were newly sequenced and assembled using both short and long reads for evolutionary analyses. Complete multi-chromosomal mitogenomes were obtained for Cypripedium subtropicum, C. henryi, Phragmipedium humboldtii, Phr. kovachii, and Paphiopedilum micranthum, and draft assemblies were obtained for four additional Paphiopedilum species. Thirty-nine protein-coding genes were annotated and shared in nine sampled species. sdh4 was discovered in all species of Cypripedioideae, and rpl10 was detected in four species of Paphiopedilum. These two genes might have been horizontally transferred from non-orchid plants at different times. Approximately 101 to 998 repeat sequences were identified with total lengths of 417,136 to 785,960 bp in the mitogenomes of Cypripedioideae. There were 634 and 662 RNA editing sites in C. subtropicum and Pa. gratrixianum, respectively, and C-to-U editing was dominant. The nad and ccm genes exhibited high frequencies of RNA editing. Our study revealed the complexity of orchid mitogenomes, including evidence for the horizontal transfer of rpl10 and sdh4.