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<p><i>Macrobrachium rosenbergii</i> is a pillar species in global freshwater crustacean aquaculture. Decapod iridescent virus 1 (DIV1) causes 60%-90% cumulative mortality in cultured ponds, leading to massive economic losses with no effective control measures. This study aimed to elucidate DIV1 relative resistance mechanisms and screen core biomarkers in <i>M. rosenbergii</i>. We constructed artificially selected DIV1-resistant (GK), susceptible (YG) and control (DZ) group from M. rosenbergii populations. After standardized DIV1 challenge, plasma samples were collected at 72 h post-infection, and analyzed via integrated DIA-MS proteomics, PRM targeted validation and bioinformatics analysis. GK group had a 2.7-fold longer median survival time (118 h vs. 44 h in YG) and 69% lower cumulative mortality risk. The core relative resistance mechanism was identified as enhanced proteostasis network plus selective metabolic reprogramming. PRM validation of 12 core DEPs showed high consistency with DIA. results (Pearson r = 0.92, P &lt; 0.001), with 5 key proteins forming a multi-dimensional antiviral network. This study first reveals a non-canonical anti-DIV1 immune paradigm in crustaceans. The screened core biomarkers provide key molecular targets for M. rosenbergii precision breeding and field early warning, laying a theoretical foundation for sustainable DIV1 control.</p>

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Integrated DIA-PRM proteomics Deciphers the molecular mechanisms and identifies core biomarkers of relative resistance to decapod iridescent Virus 1 (DIV1) in Macrobrachium rosenbergii

  • 期刊论文

  • 发表日期：

    2026年8月4日

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  • Yang Xu,
  • Chenhui Wu,
  • Yunpeng Fan,
  • Chaofan Yu,
  • Qiang Gao

摘要

Macrobrachium rosenbergii is a pillar species in global freshwater crustacean aquaculture. Decapod iridescent virus 1 (DIV1) causes 60%-90% cumulative mortality in cultured ponds, leading to massive economic losses with no effective control measures. This study aimed to elucidate DIV1 relative resistance mechanisms and screen core biomarkers in M. rosenbergii. We constructed artificially selected DIV1-resistant (GK), susceptible (YG) and control (DZ) group from M. rosenbergii populations. After standardized DIV1 challenge, plasma samples were collected at 72 h post-infection, and analyzed via integrated DIA-MS proteomics, PRM targeted validation and bioinformatics analysis. GK group had a 2.7-fold longer median survival time (118 h vs. 44 h in YG) and 69% lower cumulative mortality risk. The core relative resistance mechanism was identified as enhanced proteostasis network plus selective metabolic reprogramming. PRM validation of 12 core DEPs showed high consistency with DIA. results (Pearson r = 0.92, P < 0.001), with 5 key proteins forming a multi-dimensional antiviral network. This study first reveals a non-canonical anti-DIV1 immune paradigm in crustaceans. The screened core biomarkers provide key molecular targets for M. rosenbergii precision breeding and field early warning, laying a theoretical foundation for sustainable DIV1 control.