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<p>Heavy metal bioaccumulation in marine organisms is a critical concern for the oceanic environment and food safety. Scallops, as filter-feeding bivalves, accumulate cadmium (Cd) in their midgut gland to toxic concentrations that may pose risks to human health. Despite extensive research on Cd bioaccumulation in the Yesso scallop <i>Mizuhopecten yessoensis</i>, a major Cd-binding protein has not yet been functionally characterized. In this study, we used high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) to isolate Cd-binding proteins, and identified myCRP, a novel high-molecular-weight Cd-binding protein. myCRP contains a cysteine-rich sequence, resembling metallothionein Cd-binding motifs, and is expressed exclusively in the midgut gland. Cd-binding assays demonstrated that myCRP binds Cd in a dose-dependent manner. Immunostaining revealed the presence of a small cluster of myCRP-containing cells in the connective tissue surrounding digestive tubules in the midgut gland. Synchrotron radiation X-ray fluorescence (SR-XRF) analyses showed the distribution of Cd throughout the entire midgut gland tissue. We conclude that myCRP is partly responsible for Cd binding in the midgut gland. Our findings clarify a new mechanism of Cd-binding in marine organisms from both biological and molecular perspectives.</p>

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myCRP, Cysteine-Rich Protein in the Yesso Scallop Mizuhopecten yessoensis, Is a Novel High-Molecular Weight Cd-Binding Protein

  • 期刊论文

  • 发表日期：

    2025年12月26日

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  • Zehua Zheng,
  • Yuto Namikawa,
  • Masahiko Awaji,
  • Yugo Kato,
  • Kurin Iimura,
  • Makoto Nakakido,
  • Kouhei Tsumoto,
  • Lumi Negishi,
  • Hitoshi Kurumizaka,
  • Shino Homma-Takeda,
  • Michio Suzuki

摘要

Heavy metal bioaccumulation in marine organisms is a critical concern for the oceanic environment and food safety. Scallops, as filter-feeding bivalves, accumulate cadmium (Cd) in their midgut gland to toxic concentrations that may pose risks to human health. Despite extensive research on Cd bioaccumulation in the Yesso scallop Mizuhopecten yessoensis, a major Cd-binding protein has not yet been functionally characterized. In this study, we used high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) to isolate Cd-binding proteins, and identified myCRP, a novel high-molecular-weight Cd-binding protein. myCRP contains a cysteine-rich sequence, resembling metallothionein Cd-binding motifs, and is expressed exclusively in the midgut gland. Cd-binding assays demonstrated that myCRP binds Cd in a dose-dependent manner. Immunostaining revealed the presence of a small cluster of myCRP-containing cells in the connective tissue surrounding digestive tubules in the midgut gland. Synchrotron radiation X-ray fluorescence (SR-XRF) analyses showed the distribution of Cd throughout the entire midgut gland tissue. We conclude that myCRP is partly responsible for Cd binding in the midgut gland. Our findings clarify a new mechanism of Cd-binding in marine organisms from both biological and molecular perspectives.