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<p>Vibriosis caused by <i>Vibrio anguillarum</i> has been an important bacterial disease in cultured rainbow trout (<i>Oncorhynchus mykiss</i>). In the present study, we evaluated the protective efficacy of a vaccine that consists of formalin-killed (FK) <i>V. anguillarum</i> and the <i>alr</i> genes knockout auxotrophic-live (AL) <i>V. anguillarum</i> (Δ<i>alr1</i>Δ<i>alr2 V. anguillarum</i>). Fish were immunized with a high dose of the FK <i>V. anguillarum</i> vaccine or four different combinations of FK and AL <i>V. anguillarum</i>. In the challenge test, fish immunized with 1 × 10<sup>6</sup>&#xa0;CFU of FK <i>V. anguillarum</i> plus 1 × 10<sup>4</sup>&#xa0;CFU of AL <i>V. anguillarum</i> (FK-10<sup>6</sup> + AL-10<sup>4</sup>) showed complete protection (100% RPS) against <i>V. anguillarum</i>. In comparison, fish immunized with 1 × 10<sup>7</sup>&#xa0;CFU of FK <i>V. anguillarum</i> (FK-10<sup>7</sup>) showed much lower survival rates. In the result of ELISA, the antibody titer of fish immunized with FK-10<sup>6</sup> + AL-10<sup>4</sup> was significantly higher than that of the PBS group, but the titer was not higher than FK-10<sup>7</sup>, suggesting that the higher protection by the FK + AL combination vaccine might be mediated by not only humoral immunity but also other protective factors conferred by live bacteria in the combination vaccine. In conclusion, the present FK + AL combination vaccine efficiently protected rainbow trout with approximately 10 times lower doses of bacteria than FK-10<sup>7</sup>, which could lessen the safety problem caused by a high-tittered live bacteria vaccine and the lower immunogenicity problem of killed bacteria vaccine.</p>

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Improved Protective Efficacy in Rainbow Trout (Oncorhynchus mykiss) Against Vibrio anguillarum Through Immunization with a Combination of Formalin-Killed and Auxotrophic-Live V. anguillarum

  • 期刊论文

  • 发表日期：

    2025年1月27日

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  • Kyung Min Lee,
  • Ki Hong Kim

摘要

Vibriosis caused by Vibrio anguillarum has been an important bacterial disease in cultured rainbow trout (Oncorhynchus mykiss). In the present study, we evaluated the protective efficacy of a vaccine that consists of formalin-killed (FK) V. anguillarum and the alr genes knockout auxotrophic-live (AL) V. anguillarum (Δalr1Δalr2 V. anguillarum). Fish were immunized with a high dose of the FK V. anguillarum vaccine or four different combinations of FK and AL V. anguillarum. In the challenge test, fish immunized with 1 × 106 CFU of FK V. anguillarum plus 1 × 104 CFU of AL V. anguillarum (FK-106 + AL-104) showed complete protection (100% RPS) against V. anguillarum. In comparison, fish immunized with 1 × 107 CFU of FK V. anguillarum (FK-107) showed much lower survival rates. In the result of ELISA, the antibody titer of fish immunized with FK-106 + AL-104 was significantly higher than that of the PBS group, but the titer was not higher than FK-107, suggesting that the higher protection by the FK + AL combination vaccine might be mediated by not only humoral immunity but also other protective factors conferred by live bacteria in the combination vaccine. In conclusion, the present FK + AL combination vaccine efficiently protected rainbow trout with approximately 10 times lower doses of bacteria than FK-107, which could lessen the safety problem caused by a high-tittered live bacteria vaccine and the lower immunogenicity problem of killed bacteria vaccine.