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<p>An integrated transcriptomic and metabolomic analysis was conducted to elucidate the molecular mechanisms underlying heat stress response in <i>Pleurotus pulmonarius</i>, a commercially important edible mushroom sensitive to elevated temperatures. Through comparative RNA-seq and UPLC-MS based metabolomics, we identified 4,388 differentially expressed genes (DEGs) and 1,282 differentially accumulated metabolites (DAMs) in mycelia exposed to 42&#xa0;°C for 6&#xa0;h compared with control conditions. Integrated pathway analysis revealed that pyrimidine metabolism, tryptophan metabolism, and cysteine/methionine metabolism were coordinately reprogrammed at both transcriptional and metabolic levels, forming a synergistic regulatory network. These pathways collectively provide essential precursors for nucleic acid synthesis, modulate auxin (IAA)-mediated stress signaling, and sustain antioxidant capacity, respectively. Our findings establish a systems-level framework for understanding heat adaptation in <i>P. pulmonarius</i> and highlight key metabolic nodes that may serve as targets for molecular breeding of thermotolerant cultivars.</p>

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Integrated transcriptomic and metabolomic analysis reveals the regulatory networks in response to heat stress in Pleurotus pulmonarius

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月23日

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  • Na Lu,
  • Guanping Chen,
  • Hailong Wei,
  • Jiayao Lin,
  • Weike Wang

摘要

An integrated transcriptomic and metabolomic analysis was conducted to elucidate the molecular mechanisms underlying heat stress response in Pleurotus pulmonarius, a commercially important edible mushroom sensitive to elevated temperatures. Through comparative RNA-seq and UPLC-MS based metabolomics, we identified 4,388 differentially expressed genes (DEGs) and 1,282 differentially accumulated metabolites (DAMs) in mycelia exposed to 42 °C for 6 h compared with control conditions. Integrated pathway analysis revealed that pyrimidine metabolism, tryptophan metabolism, and cysteine/methionine metabolism were coordinately reprogrammed at both transcriptional and metabolic levels, forming a synergistic regulatory network. These pathways collectively provide essential precursors for nucleic acid synthesis, modulate auxin (IAA)-mediated stress signaling, and sustain antioxidant capacity, respectively. Our findings establish a systems-level framework for understanding heat adaptation in P. pulmonarius and highlight key metabolic nodes that may serve as targets for molecular breeding of thermotolerant cultivars.