<p>Janus vesicles, unique nanostructures, have attracted significant attention for their diverse applications in biomedical and microfluidic systems. In practical micro-nano systems, flow and electric fields often coexist, and the perforation dynamics of Janus vesicles exhibit complex motion due to their synergistic effects. Studying Janus vesicle perforation dynamics under the combined influence of fluid flow and electric fields provides valuable insights into their applications in drug delivery, catalyst delivery, and controlled release. This study focuses on the perforation dynamics and directional motion of Janus vesicles in microchannels, emphasizing how electric field strength and charge distribution on the membrane influence vesicle migration, deformation, and trajectories. Results show that when electromagnetic forces and flow-driven forces align, increasing electric field strength promotes vesicle migration and perforation. Vesicle migration is correlated with charge distribution on the membrane, with broader distributions resulting in more pronounced migration. When electric field strength remains constant, charge distribution has little effect on vesicle deformation. Conversely, when electromagnetic forces and flow-driven forces oppose, increasing electric field strength inhibits vesicle migration. At a specific potential difference, charged vesicles cease movement before reaching the perforation site, indicating the critical potential for perforation. The study also reveals that the direction of the electric field significantly affects vesicle migration direction. Adjusting potential values at microchannel boundaries can control the directional movement of Janus vesicles. This research provides new insights into Janus vesicle behavior in complex environments and deepens understanding of their potential as drug carriers for delivery and targeted therapy.</p>