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<p>Acute rejection remains a significant challenge in organ transplantation. Photobiomodulation (PBM) has demonstrated anti-inflammatory and regenerative properties, suggesting potential benefits in modulating immune responses. This study aimed to evaluate the effects of PBM on fetal intestinal transplantation in mice.​ The study was conducted in two phases. In the first phase, 77 mice were utilized to assess histological outcomes. Fetal intestines from pregnant C57BL/6 mice were transplanted into BALB/c recipients, forming allogeneic groups (ALLO-GTx) with or without PBM (ALLO-GTxPBM), and syngeneic controls (RG). PBM was applied transcutaneous at the graft site immediately post-surgery (660 nm, 40 J/cm², 1.5 J). Grafts were collected on postoperative days 3, 5, and 7 for hematoxylin-eosin staining and evaluated using Aubert’s criteria. In the second phase, spleens from 30 transplanted mice were analyzed on day 7 via flow cytometry for lymphocyte activation markers (CD4+, CD8+, CD44, CD69, CD54, CD62L).​ PBM significantly reduced rejection scores on days 5 (3.5 [3–9] vs. 12 [10–13.8]; <i>p</i> = 0.0006) and 7 (<i>p</i> = 0.0096), and enhanced graft development (<i>p</i> = 0.0079). Flow cytometry revealed decreased activation of CD4 + CD44+CD69+ (9.08% to 3.15%) and CD8 + CD44+CD69+ (1.53% to 0.90%) T cells in PBM-treated animals. Additionally, lower expression levels of CD62L + and CD54 were observed, indicating immunomodulatory effects. PBM improved graft viability and mitigated acute rejection in fetal intestinal transplantation in mice. These findings support further investigation into PBM as a therapeutic strategy to modulate immune responses in transplantation.</p>

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Photobiomodulation in acute rejection of fetal intestinal grafts: morphological aspects and lymphocyte activation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月11日

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  • Sérgio Honório,
  • Priscila Barbosa Carvalho,
  • Regiane Cristine de Souza Almeida,
  • Allan Evangelista Alves Costa,
  • Jacqueline de Fátima Jacysyn,
  • Márcia Kiyomi Koike,
  • Fernando Russo Costa do Bomfim,
  • Edna Frasson de Souza Montero

摘要

Acute rejection remains a significant challenge in organ transplantation. Photobiomodulation (PBM) has demonstrated anti-inflammatory and regenerative properties, suggesting potential benefits in modulating immune responses. This study aimed to evaluate the effects of PBM on fetal intestinal transplantation in mice.​ The study was conducted in two phases. In the first phase, 77 mice were utilized to assess histological outcomes. Fetal intestines from pregnant C57BL/6 mice were transplanted into BALB/c recipients, forming allogeneic groups (ALLO-GTx) with or without PBM (ALLO-GTxPBM), and syngeneic controls (RG). PBM was applied transcutaneous at the graft site immediately post-surgery (660 nm, 40 J/cm², 1.5 J). Grafts were collected on postoperative days 3, 5, and 7 for hematoxylin-eosin staining and evaluated using Aubert’s criteria. In the second phase, spleens from 30 transplanted mice were analyzed on day 7 via flow cytometry for lymphocyte activation markers (CD4+, CD8+, CD44, CD69, CD54, CD62L).​ PBM significantly reduced rejection scores on days 5 (3.5 [3–9] vs. 12 [10–13.8]; p = 0.0006) and 7 (p = 0.0096), and enhanced graft development (p = 0.0079). Flow cytometry revealed decreased activation of CD4 + CD44+CD69+ (9.08% to 3.15%) and CD8 + CD44+CD69+ (1.53% to 0.90%) T cells in PBM-treated animals. Additionally, lower expression levels of CD62L + and CD54 were observed, indicating immunomodulatory effects. PBM improved graft viability and mitigated acute rejection in fetal intestinal transplantation in mice. These findings support further investigation into PBM as a therapeutic strategy to modulate immune responses in transplantation.