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Introduction <p>Photodynamic therapy (PDT) offers broad-spectrum antifungal effects with lower resistance in some studies. This study evaluates the effectiveness of two PDT protocols, using a 660&#xa0;nm diode laser with methylene blue (MB) compared to an 810&#xa0;nm diode laser with indocyanine green (ICG), in reducing colony counts of <i>Candida albicans</i>,<i> C. glabrata</i>,<i> C. tropicalis</i>,<i> and C. krusei</i>.</p> Methods <p>This experimental laboratory study tested 4 standard <i>Candida</i> species in seven groups (2 PDT, 2 photosensitizers alone, 2 lasers wavelength with saline, 1 control with saline) each containing five replicates per species. PDT was performed using MB with a 660&#xa0;nm diode laser and ICG with an 810&#xa0;nm diode laser. Laser exposure setting was 100 mW, 10&#xa0;J/cm², 100&#xa0;s. CFUs were quantified, and statistical analysis was performed using SPSS version 26, with significance set at <i>P</i> &lt; 0.05.</p> Results <p>PDT had better efficacy for colony count reduction of C. tropicalis, and C. krusei. PDT with an 810&#xa0;nm diode laser and ICG had better efficacy than PDT with 660&#xa0;nm diode laser and MB but difference was not significant (<i>P</i> &gt; 0.05). For some candida species there was no significant difference between PDT with laser alone for colony reduction but there was significant difference between them with each photosensitizer and saline alone (<i>P</i> &lt; 0.05).</p> Conclusion <p>Both PDT had better efficacy for colony count reduction of C. tropicalis, and C. krusei compare to other two species. PDT with diode 810&#xa0;nm and ICG seems better than PDT with diode 660&#xa0;nm and MB, but the difference was not statistical between two methods.</p>

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Comparison of PDT using a 660 nm laser and methylene blue vs. an 810 nm laser and indocyanine green on different Candida species – in vitro

  • 期刊论文

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  • 发表日期：

    2026年1月31日

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  • Masoume Norouzbeigi,
  • Amir Mansour Shirani,
  • Arezoo Tahmourespour

摘要

Introduction

Photodynamic therapy (PDT) offers broad-spectrum antifungal effects with lower resistance in some studies. This study evaluates the effectiveness of two PDT protocols, using a 660 nm diode laser with methylene blue (MB) compared to an 810 nm diode laser with indocyanine green (ICG), in reducing colony counts of Candida albicans, C. glabrata, C. tropicalis, and C. krusei.

Methods

This experimental laboratory study tested 4 standard Candida species in seven groups (2 PDT, 2 photosensitizers alone, 2 lasers wavelength with saline, 1 control with saline) each containing five replicates per species. PDT was performed using MB with a 660 nm diode laser and ICG with an 810 nm diode laser. Laser exposure setting was 100 mW, 10 J/cm², 100 s. CFUs were quantified, and statistical analysis was performed using SPSS version 26, with significance set at P < 0.05.

Results

PDT had better efficacy for colony count reduction of C. tropicalis, and C. krusei. PDT with an 810 nm diode laser and ICG had better efficacy than PDT with 660 nm diode laser and MB but difference was not significant (P > 0.05). For some candida species there was no significant difference between PDT with laser alone for colony reduction but there was significant difference between them with each photosensitizer and saline alone (P < 0.05).

Conclusion

Both PDT had better efficacy for colony count reduction of C. tropicalis, and C. krusei compare to other two species. PDT with diode 810 nm and ICG seems better than PDT with diode 660 nm and MB, but the difference was not statistical between two methods.