Scholarlink

<p>Several parameters influence the effectiveness of photobiomodulation therapy (PBMT) in improving skin flap viability, yet the role of the number of treatment sessions remains underexplored. The present study aimed to evaluate the effect of different numbers of laser PBMT sessions on skin flap viability. Thirty-two Wistar rats were randomly divided into four groups: G1 (PBMT simulation), G2 (PBMT for 2 consecutive days), G3 (PBMT for 5 days), and G4 (PBMT for 7 consecutive days). Treatment began immediately after surgery with the following parameters: GaAlAs diode laser, 660 nm wavelength, continuous mode, spot size of 0.04 cm² (probe in contact with the skin), 90 J/cm² fluence, 40 mW output power, 90 s application time, and 3.6 J of energy per point with irradiation at three points and 24-hour intervals between sessions according to each group’s protocol. On the 7th postoperative day, tissue was collected from the irradiated area for analysis of necrotic area, vessel and mast cell morphometry and immunohistochemistry for angiogenesis markers. G2 showed the smallest necrotic area and a higher percentage of VEGF- and HIF-1α-positive cells compared to G1. Short PBMT protocols improved flap viability versus controls and produced outcomes comparable to extended regimens, supporting relevance for translational research.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Number of sessions of applications of laser photobiomodulation therapy interferes with the viability of skin flap: an experimental study in rats

  • 期刊论文

  • 发表日期：

    2025年12月29日

查看全文
  • Bruno Batista,
  • Cintia Cristina Santi Martignago,
  • Homero Garcia-Motta,
  • Bruna Nascimento,
  • Carla Roberta Tim,
  • Livia Assis,
  • Richard Eloin Liebano,
  • Nivaldo Antonio Parizotto

摘要

Several parameters influence the effectiveness of photobiomodulation therapy (PBMT) in improving skin flap viability, yet the role of the number of treatment sessions remains underexplored. The present study aimed to evaluate the effect of different numbers of laser PBMT sessions on skin flap viability. Thirty-two Wistar rats were randomly divided into four groups: G1 (PBMT simulation), G2 (PBMT for 2 consecutive days), G3 (PBMT for 5 days), and G4 (PBMT for 7 consecutive days). Treatment began immediately after surgery with the following parameters: GaAlAs diode laser, 660 nm wavelength, continuous mode, spot size of 0.04 cm² (probe in contact with the skin), 90 J/cm² fluence, 40 mW output power, 90 s application time, and 3.6 J of energy per point with irradiation at three points and 24-hour intervals between sessions according to each group’s protocol. On the 7th postoperative day, tissue was collected from the irradiated area for analysis of necrotic area, vessel and mast cell morphometry and immunohistochemistry for angiogenesis markers. G2 showed the smallest necrotic area and a higher percentage of VEGF- and HIF-1α-positive cells compared to G1. Short PBMT protocols improved flap viability versus controls and produced outcomes comparable to extended regimens, supporting relevance for translational research.